Human Tripeptidyl-Peptidase I/TPP1 Alexa Fluor™ Plus 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2237AFP594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 254008
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tripeptidyl‑Peptidase I/TPP1
Specificity
Detects human Tripeptidyl‑Peptidase I/TPP1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Tripeptidyl-Peptidase I/TPP1
Tripeptidyl-Peptidase I (TPP1), also known as tripeptidyl aminopeptidase, is a lysosomal peptidase which can hydrolyze tripeptides from the N-termini of oligopeptides and also possesses weak endopeptidase activity (1‑3). TPP1 is a serine peptidase with a Ser-Glu-Asp catalytic triad, making it a member of the sedolisin family (4). The TPP1 precursor undergoes autoactivation under conditions of acidic pH (4). TPP1 is expressed in many tissues, with elevated expression in tissues associated with peptide hormone production (5). Mutations in TPP1 have been shown to be a cause of classic late‑infantile neuronal ceroid lipofuscinosis (CLN2), a lysosomal storage disease (6).
References
- Sleat, D.E. et al. (1997) Science 277:1802.
- Ezaki, J. et al. (1999) J. Neurochem. 72:2573.
- Tomkinson, B. (1999) Trends Biochem. Sci. 24:355.
- Lin, L. et al. (2001) J. Biol. Chem. 276:2249.
- Kida, E. et al. (2001) J. Neuropathol. Exp. Neurol. 60:280.
- Sleat, D.E. et al. (1999) Am. J. Hum. Genet. 64:1511.
Alternate Names
CLN2, LINCL, LPIC, TPP1, TripeptidylPeptidase I
Entrez Gene IDs
1200 (Human)
Gene Symbol
TPP1
UniProt
Additional Tripeptidyl-Peptidase I/TPP1 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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