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Human TRAIL R2/TNFRSF10B Antibody
Catalog # MAB631 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Agonist Activity
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 71903
Product Summary for Human TRAIL R2/TNFRSF10B Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
Met1-Glu182
Accession # CAG46696
Met1-Glu182
Accession # CAG46696
Specificity
Detects human TRAIL R2/TNFRSF10B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R3, rhTRAIL R4, or rhDcR3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TRAIL R2/TNFRSF10B Antibody
Human TRAIL R2/TNFRSF10B Antibody Induces Apoptosis of Jurkat Cells.
Human TRAIL R2/TNFRSF10B Monoclonal Antibody induces apoptosis of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner, as measured by Resazurin (Catalog # AR002) . The ED50 for this effect is typically 2-12 ng/mL.Applications for Human TRAIL R2/TNFRSF10B Antibody
Application
Recommended Usage
Agonist Activity
2-12 ng/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 8 publications using MAB631 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R2/TNFRSF10B
Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.
Product Documents for Human TRAIL R2/TNFRSF10B Antibody
Product Specific Notices for Human TRAIL R2/TNFRSF10B Antibody
For research use only
Citations for Human TRAIL R2/TNFRSF10B Antibody (8)
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Citations are publications that use Bio-Techne products. Selected citations for Human TRAIL R2/TNFRSF10B Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Functional AssayKang Z et al. (2015-08-20), Caspase-8 expression is predictive of tumour response to death receptor 5 agonist antibody in Ewing's sarcoma. Br J Cancer, 2015-0113(6):894-901.
PMID: 26291055 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayBozza et al. (2014-09-04), The use of a stably expressed FRET biosensor for determining the potency of cancer drugs. PLoS ONE, 2014-09(9):e107010.
PMID: 25188024 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayMeng X et al. (2014-07-25), Poly(ADP-ribose) polymerase inhibitors sensitize cancer cells to death receptor-mediated apoptosis by enhancing death receptor expression. J Biol Chem, 2014-0289(30):20543-58.
PMID: 24895135 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayMatte I et al. (2014-04-01), MUC16 mucin (CA125) attenuates TRAIL-induced apoptosis by decreasing TRAIL receptor R2 expression and increasing c-FLIP expression. BMC Cancer, 2014-014(0):234.
PMID: 24690311 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayKang Z et al. (2011-03-08), Drozitumab, a human antibody to death receptor 5, has potent antitumor activity against rhabdomyosarcoma with the expression of caspase-8 predictive of response. Clin Cancer Res, 2011-017(10):3181-92.
PMID: 21385927 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PStary G et al. (2009-08-18), Plasmacytoid dendritic cells express TRAIL and induce CD4+ T-cell apoptosis in HIV-1 viremic patients. Blood, 2009-0114(18):3854-63.
PMID: 19690337 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayLocklin RM et al. (2007-12-07), Selective targeting of death receptor 5 circumvents resistance of MG-63 osteosarcoma cells to TRAIL-induced apoptosis. Mol. Cancer Ther., 2007-16(12):3219-28.
PMID: 18065493 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayShigeno M et al. (2003-03-20), Interferon-alpha sensitizes human hepatoma cells to TRAIL-induced apoptosis through DR5 upregulation and NF-kappa B inactivation. Oncogene, 2003-022(11):1653-62.
PMID: 12642868
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