Human TRAIL R2/TNFRSF10B Antibody

Catalog # MAB6313 | R&D Systems, Inc. a Bio-Techne Brand
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MAB6313
MAB6313-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 603307

Product Summary for Human TRAIL R2/TNFRSF10B Antibody

Immunogen

E. coli-derived recombinant human TRAIL R2/TNFRSF10B
Ile56-Pro128
Accession # NP_003833

Specificity

Detects human TRAIL R2/TNFRSF10B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhOPG, rhRANK, rhRELT, rhTNF RI, rhTNF RII, rhTRAIL R1, rhTRAIL R3, rhTRAIL R4, or rhTWEAK R is observed. Additionally in direct ELISAs, no cross‑reactivity with rhTRAIL R4, rhXEDAR, recombinant mouse (rm) DR5, rhTRAIL R1, rmTRAIL R2, or rmTNFRH3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TRAIL R2/TNFRSF10B Antibody

Detection of Human TRAIL R2/TNFRSF10B antibody by Western Blot.

Detection of Human TRAIL R2/TNFRSF10B by Western Blot.

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB6313) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TRAIL R2/TNFRSF10B at approximately 44 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human TRAIL R2/TNFRSF10B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HCT‑116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB6313 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1 membrane protein of the TNF R family, designated TNFRSF10B, which is a receptor for TRAIL (APO2 ligand). The human TRAIL R2 cDNA encodes a 440 amino acid (aa) precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% aa sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References

  1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
  2. Walczak, H. et al. (1997) EMBO J. 16:5386.
  3. Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Gene Symbol

TNFRSF10B

UniProt

NP_003833

Product Documents for Human TRAIL R2/TNFRSF10B Antibody

Certificate of Analysis

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Product Specific Notices for Human TRAIL R2/TNFRSF10B Antibody

For research use only

Customer Reviews for Human TRAIL R2/TNFRSF10B Antibody (1)

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  • Human TRAIL R2/TNFRSF10B Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 07/23/2018
    MDA-MB-231 cells were treated with CHX (20 ug/ml) in indicated time points. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human TRAIL R2 Antibody (MAB6313). A specific band was detected for TRAIL R2 at approximately 45-55 kDa. This experiment was conducted under reducing conditions
    Human TRAIL R2/TNFRSF10B Antibody MAB6313

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