Key Product Details
Product Summary for Human TRAIL R2/TNFRSF10B Antibody
Accession # NP_003833
Scientific Data Images for Human TRAIL R2/TNFRSF10B Antibody
Detection of Human TRAIL R2/TNFRSF10B by Western Blot.Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB6313) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TRAIL R2/TNFRSF10B at approximately 44 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Applications for Human TRAIL R2/TNFRSF10B Antibody
Sample: HCT‑116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
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Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R2/TNFRSF10B
Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1 membrane protein of the TNF R family, designated TNFRSF10B, which is a receptor for TRAIL (APO2 ligand). The human TRAIL R2 cDNA encodes a 440 amino acid (aa) precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% aa sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.
Product Specific Notices for Human TRAIL R2/TNFRSF10B Antibody
For research use only