Human TIMP-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB973
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
ELISA Detection, Immunocytochemistry, Immunohistochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 183551
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-3
Cys24-Pro211
Accession # P35625
Cys24-Pro211
Accession # P35625
Specificity
Detects human TIMP-3 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human TIMP-1, -2, or -4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TIMP-3 Antibody
Detection of TIMP-3 in Human Breast Tumor.
TIMP-3 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Mouse Anti-Human TIMP-3 Monoclonal Antibody (Catalog # MAB973) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of tumor cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human TIMP-3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TIMP-3 (Catalog # 973-TM), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human TIMP-3 (Catalog # 973-TM), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human TIMP-3 (Catalog # 973-TM)
Sample: Recombinant Human TIMP-3 (Catalog # 973-TM)
Human TIMP-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 1 using MAB973 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-3
Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-3 is a glycoprotein with a molecular mass of 30 kDa produced by a wide range of cell types. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, TIMP-3 is the only known member of the TIMP family that is an effective inhibitor of ADAMs such as TACE (1).
TIMP-3 is unique among the TIMPs because of its high affinity for binding to the extracellular matrix (2). Point mutations in the TIMP-3 C-terminal domain have been reported to result in Sorsby's fundus dystrophy, a disease leading to macular degeneration and loss of vision.
References
- Amour, A. et al. (1998) FEBS Lett. 435:39.
- Leco, K.J. et al. (1994) J. Biol. Chem. 269:9352.
Long Name
Tissue Inhibitors of Metalloproteinases 3
Alternate Names
TIMP3
Gene Symbol
TIMP3
UniProt
Additional TIMP-3 Products
Product Documents for Human TIMP-3 Antibody
Product Specific Notices for Human TIMP-3 Antibody
For research use only
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