Human TIGIT Antibody

Detection of TIGIT by Flow Cytometry

DNAM-1 Blockade Attenuates and TIGIT Blockade Augments NK-Mediated Cytotoxicity against Adenovirus-Infected CellsThe changes of NK receptors on pNK after exposure to adenovirus-infected TOV21G or their conditioned media were evaluated by flow cytometry. (A) Summary analyses of independent experiments (n = 5) for DNAM-1 (left) and TIGIT (right) expression. (B) Representative images of DNAM-1 (left) and TIGIT (right) expression (Mann-Whitney U tests; CC, co-culture; CM, conditioned-medium). pNK were co-cultured with dl922-947-infected cells with or without a blocking anti-DNAM-1 antibody (10 μg/mL) and assessed by time-lapse microscopy. (C) Representative data of fluorescent object counts over time for TOV21G (left) and summary data (n = 5) of TAUC for TOV21G (right) and (D) OVCAR4 (n = 3) are shown (one-way ANOVA with multiple comparisons test). (E) pNK were co-cultured with OV-infected TOV21G cells with or without a blocking anti-TIGIT antibody (20 μg/mL) and assessed by time-lapse microscopy (n = 5, each line represents a unique donor; also see Figure S8). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32195317), licensed under a CC-BY license. Not internally tested by R&D Systems.