Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 460904

View all TAFA3/FAM19A3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TAFA3/FAM19A3
Ala31-Arg133
Accession # Q7Z5A8

Specificity

Detects human TAFA3/FAM19A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 2, 4, or 5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TAFA3/FAM19A3 Antibody

Detection of TAFA3/FAM19A3 antibody in A172 Human Cell Line antibody by Flow Cytometry.

Detection of TAFA3/FAM19A3 in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Mouse Anti-Human TAFA3/FAM19A3 Monoclonal Antibody (Catalog # MAB4677, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human TAFA3/FAM19A3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human TAFA3/FAM19A3 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAFA3/FAM19A3

TAFA3 (also FAM19A3) is an 11 kDa (predicted) member of the FAM19/TAFA family of 5 proteins that show differential expression in the brain. It is probably a secreted protein. TAFA 1‑4 proteins bear chemokine-like signatures, and are distantly related to MIP-1 alpha, sharing its CC motif. A conserved 10 cysteine pattern suggests multiple disulfide linkages. Mature human TAFA3 is 103 aa in length and has no predicted N‑linked glycosylation sites. It shares 86% aa identity with mouse TAFA3. A potential 131 aa isoform diverges at aa 59 of the mature sequence.

Long Name

Family with Sequence Similarity 19, Member A3

Alternate Names

FAM19A3

Entrez Gene IDs

284467 (Human); 329731 (Mouse)

Gene Symbol

TAFA3

UniProt

Q7Z5A8 (Human)

Additional TAFA3/FAM19A3 Products

Product Documents for Human TAFA3/FAM19A3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TAFA3/FAM19A3 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.