Human SOX11 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11734
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1106960
Product Specifications
Immunogen
E. coli - derived recombinant human SOX-11
Met1-Pro173
Accession # P35716
Met1-Pro173
Accession # P35716
Specificity
Detects recombinant human SOX11 protein in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SOX11 Antibody
SOX11 in Human Mantle Cell Lymphoma via seqIF™ staining on COMET™
SOX11 was detected in immersion fixed paraffin-embedded sections of human Mantle Cell Lymphoma using Mouse Anti-Human SOX11, Monoclonal Antibody (Catalog # MAB11734) at 25ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.
Detection of SOX11 in Human Glioblastoma.
SOX11 was detected in immersion fixed paraffin-embedded sections of human glioblastoma using Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of SOX11 in human Mantle Cell Lymphoma
SOX11 was detected in immersion fixed paraffin-embedded sections of human mantle cell lymphoma using Mouse Anti-Human SOX11 Monoclonal Antibody (Catalog # MAB11734) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human SOX11 Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human glioblastoma and mantle cell lymphoma
Sample: Immersion fixed paraffin-embedded sections of human glioblastoma and mantle cell lymphoma
Western Blot
2 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line
Sample: SH-SY5Y human neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX11
Human SOX11 (SRY box-related gene 11) is a 60-72 kDa (46.6 kDa predicted), group C member of the SOX family of transcription factors. It is 441 amino acids (aa) in length, and contains one HMG box (aa 49-117), two poly-Gly regions (aa 144-214), one poly-Asp domain (aa 223-233) and one poly-Ser region. The HMG box determines DNA and transcriptional coactivator binding, the poly-Ser region lies in a potent transactivating region, and the poly-Asp domain autoinhibits/regulates activities of the other two regions. SOX11 appears early in development in multiple tissues, and impacts neuron, oligodendrocyte and cartilage formation. Human SOX11 is 94% aa identical to mouse SOX11 over aa 1-173. Mouse SOX11, however, is absent a 12 aa poly-Gly sequence that is present in human SOX11 between aa 145-156. Additional band correspondent to the lower molecular weight may represent SOX11 without posttranslational modifications or human SOX4, over aa 38-123 human SOX11 shares 100% aa identity with human SOX4. (1)
References
- Dictor, M. et al. (2009) Haematologica 94:1563.
Long Name
Transcription Factor SRY (Sex Determining Region Y)-box 11
Alternate Names
SRY (sex determining region Y)-box 11, SRY (sex-determining region Y)-box 11, SRY-related HMG-box gene 11, transcription factor SOX-11
Gene Symbol
SOX11
UniProt
Additional SOX11 Products
Product Documents for Human SOX11 Antibody
Product Specific Notices for Human SOX11 Antibody
For research use only
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