Human Siglec-10 Antibody

Name: Human Siglec-10 Antibody
Brand: R&D Systems
SKU: AF2130
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2130

Citations (3)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Siglec-10 Met17-Thr546 Accession # Q96LC7
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Siglec‑10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human (rh) Siglec-5 is observed and less than 1% cross-reactivity with rhSiglec-2, rhSiglec-3, rhSiglec-7, rhSiglec-9, and recombinant mouse Siglec-F is observed.

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Applications for Human Siglec-10 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Siglec‑10 Fc Chimera (Catalog # 2130-SL)
Flow Cytometry	2.5 µg/10⁶ cells	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 1.5 μg/mL of biotinylated 6’‑Sialyllactose-Polyacrylamide to immobilized Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL) coated at 5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Siglec-10 Antibody

	Flow Cytometry Detection of Siglec‑10 in Human B Cells by Flow Cytometry. Human whole blood CD19⁺B cells were stained with Goat Anti-Human Siglec-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2130, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phy-coerythrin-conjugated Anti-Goat IgG Secondary Anti-body (Catalog # F0107).

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Siglec-10

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5‑11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1‑3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V‑type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5‑8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5‑7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is up‑regulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (7). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).

Item	Value
References	Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83. Angata, T. et al. (2001) J. Biol. Chem. 276:45128. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900. Li, N. et al. (2001) J. Biol. Chem. 276:28106. Munday, J. et al. (2001) Biochem. J. 355:489. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355. Aizawa, H. et al. (2003) Genomics 82:521. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
Long Name	Sialic Acid Binding Ig-like Lectin 10
Entrez Gene IDs	89790 (Human)
Alternate Names	MGC126774, PRO940, SLG2, SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7, Siglec-10, Siglec-like protein 2, Siglec10, sialic acid binding Ig-like lectin 10, sialic acid-binding Ig-like lectin 10, siglec-like gene 2

Citations for Human Siglec-10 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Siglec-10 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

M Vijayan et al. (2020), Decidual glycodelin-A polarizes human monocytes towards a decidual macrophage-like phenotype via siglec-7 J. Cell. Sci., 0(0).
PMID: 32513821
Species: Porcine
Sample Types: Whole Cells
Applications: ICC

J Xie et al. (2018), Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells Vet. Res., 49(1):67.
PMID: 30021620
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Yokoi H et al. (2006), Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells. Allergy, 61(6):769-76.
PMID: 16677248

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Customer Reviews for Human Siglec-10 Antibody (2)

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Human Siglec-10 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Melanoma tissue

Species: Human

Verified Customer | Posted 11/04/2020
Human Siglec-10 Antibody

Name: cong xu

Application: B/N

Sample Tested: NK cell

Species: Human

Verified Customer | Posted 03/13/2020

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Human Siglec-10 Antibody

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Applications for Human Siglec-10 Antibody

Scientific Data Examples for Human Siglec-10 Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: Siglec-10

Citations for Human Siglec-10 Antibody (3)

Customer Reviews for Human Siglec-10 Antibody (2)

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Human Siglec-10 Antibody

Human Siglec-10 Antibody

Product Documents for Human Siglec-10 Antibody

Product Datasheets

COA

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