Recombinant Human Siglec-10 Fc Chimera Protein, CF Best Seller

Name: Recombinant Human Siglec-10 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 2130-SL
Rating: 5 (1 reviews)

Catalog # 2130-SL | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (3)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q96LC7
Structure / Form:	Disulfide-linked heterodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Human Siglec-10 Fc Chimera Protein, CF

Technical Data
Citations (3) / Reviews (1)

Product Specifications for Recombinant Human Siglec-10 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human Siglec-10 protein

Human Siglec-10 (Met17-Thr546) Accession # Q96LC7	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met17

Predicted Molecular Mass

85 kDa (monomer)

SDS-PAGE

85 kDa (monomer)

Activity

Measured by its ability to bind biotinylated 6’-Sialyllactose-Polyacrylamide in functional ELISA. Whitney, G. et al. (2001) FEBS 268:6083. rhSiglec-10/Fc Chimera immobilized at 5 µg/mL (100 µL/well) on a goat anti-human IgG Fc antibody (Catalog # G-102-C) coated plate can bind biotinylated 6’-Sialyllactose-Polyacrylamide with a linear range of 0.04-2.5 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2130-SL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Siglec-10

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1-3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V-type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5-8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5-7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is upregulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell.(7) When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).

Item	Value
References	Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83. Angata, T. et al. (2001) J. Biol. Chem. 276:45128. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900. Li, N. et al. (2001) J. Biol. Chem. 276:28106. Munday, J. et al. (2001) Biochem. J. 355:489. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355. Aizawa, H. et al. (2003) Genomics 82:521. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
Long Name	Sialic Acid Binding Ig-like Lectin 10
Entrez Gene IDs	89790 (Human)
Alternate Names	MGC126774, PRO940, SLG2, SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7, Siglec-10, Siglec-like protein 2, Siglec10, sialic acid binding Ig-like lectin 10, sialic acid-binding Ig-like lectin 10, siglec-like gene 2

Citations for Recombinant Human Siglec-10 Fc Chimera Protein, CF (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Siglec-10 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Natural Protein
Applications: Bioassay

J Terävä et al. (2019-07-25), Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 2019-014(7):e0219480.
PMID: 31344060
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay

Chen et al. (2014-09-03), Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1. Elife, 2014-03(0):e04066.
PMID: 25187624
Species: Human, Mouse
Sample Types: Recombinant Protein, Whole Cells
Applications: Binding Assay, Immunoprecipitation

Chen GY et al. (2011-04-10), Amelioration of sepsis by inhibiting sialidase-mediated disruption of the CD24-SiglecG interaction. Nat. Biotechnol., 2011-029(5):428-35.
PMID: 21478876

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Customer Reviews for Recombinant Human Siglec-10 Fc Chimera Protein, CF (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Human Siglec-10 Fc Chimera Protein, CF

Name: Jenna Nguyen

Application: In vivo study

Verified Customer | Posted 10/17/2019

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

Product Documents for Recombinant Human Siglec-10 Fc Chimera Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS