Human sFRP-4 Antibody

Detection of Human sFRP‑4 by Western Blot.

Western blot shows lysates of OVCAR-3 human ovarian carcinoma cell line, human ovarian cancer tissue, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human sFRP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1827) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for sFRP-4 at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of sFRP-4 by Western Blot

shRNA knockdown of gastric cancer cell lines. shRNA based lentiviral constructs were used to knockdown SFRP4 expression in (a) AGS, b SNU-1 and (c) NCI-N87 gastric cell lines using the SFRP4#3 construct and validated by Western blot. d-f Quantitation of knockdown was determined using Image J (g-i) Invasion assays were performed using WT cells, WT cells + scramble and WT cells + SFRP4 lentivirus. Reduction of SFRP4 expression resulted in a significant reduction in invasive capabilities (compared to scramble controls) in all cell lines. Pre-incubation of the cells with 20 nM recombinant human SFRP4 was able to restore the invasive ability of the knockdown cells to wild-type levels and, in the case of AGS and NCI-N87 cell lines was able to enhance the invasive capability of WT cells. Data show means ± SD. of at least three independent experiments. *p < 0.05, **p < 0.01 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33277667), licensed under a CC-BY license. Not internally tested by R&D Systems.