Human sFRP-4 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1827AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human sFRP-4
Specificity
Detects human sFRP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) sFRP-4 is observed, and less than 5% cross-reactivity with recombinant human (rh) sFRP-3, rhsFRP-1, and rmsFRP-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human sFRP-4 Alexa Fluor™ Plus 405-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: sFRP-4
sFRP-4, also known as DDC-4, Frp, FRPHE and Secreted Frizzled Related Protein 4, is a 50-55 kDa glycoprotein expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (7). Of all the secreted frizzled related proteins, sFRP-4 is most closely related to sFRP-3 (1). Mouse and human sFRP-4 proteins share 92% aa identity.
References
- Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
- Wolf, V. et al. (1997) FEBS Letters 417:385.
- Drake, J.M. et al. (2003) Apoptosis 8:389.
- James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
- Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
- Abu-Jawdeh, G. et al. (1999) Lab Investigation 79:439.
- Wong, S.C.C. et al. (2002) J. Pathology 196:145.
- Berndt, T. et al. (2003) J. Clin. Invest. 112:785.
Long Name
Secreted Frizzled Related Protein 4
Alternate Names
DDC-4, FRP-AP, frpHE, FrzB2, sFRP4
Gene Symbol
SFRP4
UniProt
Additional sFRP-4 Products
Product Specific Notices for Human sFRP-4 Alexa Fluor™ Plus 405-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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