Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Serpin D1/Heparin Cofactor II Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin D1/Heparin Cofactor II
Gly20-Ser499
Accession # P05546

Specificity

Detects human Serpin D1/Heparin Cofactor II in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Serpin D1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Serpin D1/Heparin Cofactor II Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin D1/
Heparin Cofactor II (Catalog # 3198-PI), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI)

Neutralization

Measured by its ability to neutralize Recombinant Human Serpin D1 (0.56 µg/mL, Catalog # 3198-PI) inhibition of Recombinant Human Coagulation Factor II/Thrombin (0.05 µg/mL, Catalog # 1473-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 6.6 µg/mL.

Reviewed Applications

Read 1 review rated 4 using AF3198 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin D1/Heparin Cofactor II

Serpin D1, also known as Heparin Cofactor II, is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). The full-length human cDNA was expressed and the purified protein corresponded to the secreted form with anti-thrombin activity.

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
  3. Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.

Alternate Names

HC2, HCF2, Heparin Cofactor II, LS2

Entrez Gene IDs

3053 (Human); 15160 (Mouse)

Gene Symbol

SERPIND1

UniProt

P05546

Additional Serpin D1/Heparin Cofactor II Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin D1/Heparin Cofactor II Antibody

For research use only

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