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Recombinant Human Serpin D1/Heparin Cofactor II Protein, CF

Bio-Techne includes R&D Systems | Catalog # 3198-PI

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3198-PI-010

Key Product Details

Source

Sf 21 (stably transfected)

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived human Serpin D1/Heparin Cofactor II protein
Gly20-Ser499, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly20

Predicted Molecular Mass

56 kDa

SDS-PAGE

62 kDa, reducing conditions

Activity

Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE) cleavage of a fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
The IC50 value is <1.5 nM, as measured under the described conditions.

Formulation, Preparation and Storage

3198-PI
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Serpin D1/Heparin Cofactor II

Serpin D1, also known as Heparin Cofactor II, is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). The full‑length human cDNA was expressed and the purified protein corresponded to the secreted form with anti‑thrombin activity.

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
  3. Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.

Alternate Names

HC2, HCF2, Heparin Cofactor II, LS2

Entrez Gene IDs

3053 (Human); 15160 (Mouse)

Gene Symbol

SERPIND1

UniProt

Additional Serpin D1/Heparin Cofactor II Products

Product Documents for Recombinant Human Serpin D1/Heparin Cofactor II Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Serpin D1/Heparin Cofactor II Protein, CF

For research use only

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