Detects human Semaphorin 4D/CD100 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 4B, 4C, 4G, or recombinant mouse Semaphorin 4D is observed. In Western blots, no cross-reactivity with rhSemaphorin 4A, 6A, or 6C is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Semaphorin 4D/CD100 PE-conjugated Antibody

Detection of Semaphorin 4D/CD100 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Semaphorin 4D/CD100 PE-conjugated Monoclonal Antibody (Catalog # FAB74701P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Semaphorin 4D/CD100 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 4D/CD100

Semaphorin 4D (Sema4D)/CD100, previously Sem J, G or C-like 2, is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Semaphorin 4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Semaphorin 4D shares 87% and 93% aa identity with human and rat Semaphorin 4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Semaphorin 4D binds CD72 on B and dendritic cells and Plexin B1 elsewhere. In developing neurons, Semaphorin 4D assists in guidance. Semaphorin 4D is proangiogenic when expressed by tumor-associated macrophages.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Entrez Gene IDs

10507 (Human); 20354 (Mouse); 306790 (Rat)

Gene Symbol

SEMA4D

UniProt

Q92854

Additional Semaphorin 4D/CD100 Products

Product Documents for Human Semaphorin 4D/CD100 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Semaphorin 4D/CD100 PE-conjugated Antibody

For research use only

Citations
Reviews

Human Semaphorin 4D/CD100 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB74701P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Semaphorin 4D/CD100 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Semaphorin 4D/CD100 PE-conjugated Antibody

Detection of Semaphorin 4D/CD100 in Jurkat Human Cell Line by Flow Cytometry.

Applications for Human Semaphorin 4D/CD100 PE-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: Semaphorin 4D/CD100

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Semaphorin 4D/CD100 Products

Product Documents for Human Semaphorin 4D/CD100 PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human Semaphorin 4D/CD100 PE-conjugated Antibody