Human S100A8 Antibody

Name: Human S100A8 Antibody
Brand: R&D Systems
SKU: AF4570
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF4570

Citations (2)

(1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human S100A8 Met1-Glu93 Accession # P05109
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Sheep IgG
Specificity:	Detects human S100A8 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant mouse S100A8, recombinant human (rh) S100A1, and rhS100P is observed.

View Full Technical Details for Human S100A8 Antibody

Applications for Human S100A8 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below
Intracellular Staining by Flow Cytometry	2.5 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human S100A8 Antibody

	Western Blot Detection of Human S100A8 by Western Blot. Western blot shows lysates of human brain tissue, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4570) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
	Immunocytochemistry S100A8 in HeLa Human Cell Line. S100A8 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4570) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Intracellular Staining by Flow Cytometry Detection of S100A8 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4570, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
	Simple Western Detection of Human S100A8 by Simple Western^TM. Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A8 at approximately 10 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4570) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: S100A8

S100A8 (also known as MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca‑dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12-47 and 46-81) and one high-affinity Ca‑binding site (aa 59-70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP‑2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Item	Value
Long Name	S100 Calcium Binding Protein A8
Entrez Gene IDs	6279 (Human); 20201 (Mouse)
Alternate Names	60B8AG, CAGACP-10, CFAG, CFAGL1Ag, CGLA, Calgranulin A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MA387, MIF, MRP-8, MRP8, MRP8S100 calcium binding protein A8 (calgranulin A), Migration inhibitory factor-related protein 8, NIF, P8, S100 calcium binding protein A8, S100 calcium-binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, Urinary stone protein band A, calgranulin-A, protein S100-A8

Citations for Human S100A8 Antibody (2)

Showing 1 - 2 of 2 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human S100A8 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

S Sahu et al. (2020), InsP7 is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 32727897
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

S Sahu et al. (2020), InsP7 is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 32727897

There are no citations that match your criteria.

Showing 1 - 2 of 2 citations Showing All

Customer Reviews for Human S100A8 Antibody (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Human S100A8 Antibody

Name: Anonymous

Application: WB

Sample Tested: Recombinant protein

Species: Human

Verified Customer | Posted 07/01/2016

S100A8 Antibody tested against different concentrations of human recombinant S100A8/A9.

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

Product Documents for Human S100A8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human S100A8 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Human S100A8 Antibody

Scientific Data Examples for Human S100A8 Antibody

Western Blot

Immunocytochemistry

Intracellular Staining by Flow Cytometry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: S100A8

Citations for Human S100A8 Antibody (2)

Customer Reviews for Human S100A8 Antibody (1)

Customer Images

Human S100A8 Antibody

Product Documents for Human S100A8 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected