Human S100A8 Antibody

Name: Human S100A8 Antibody
Brand: R&D Systems
SKU: MAB4570
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB4570

Citations (4)

(1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human S100A8 Met1-Glu93 Accession # P05109
Applications:	Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 749916
Specificity:	Detects human S100A8 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) S100A8/A9 is observed and no cross-reactivity with rhS100A9, recombinant mouse (rm) S100A8, rmS100A9, or rmS100A8/A9 is observed.

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Applications for Human S100A8 Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Immunohistochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human S100A8 Antibody

	Western Blot Detection of Human S100A8 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A8 Monoclonal Antibody (Catalog # MAB4570) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A8 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry S100A8 in Human Lung Cancer Tissue. S100A8 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human S100A8 Monoclonal Antibody (Catalog # MAB4570) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Sterile PBS to a final concentration of 0.5 mg/mL. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: S100A8

S100A8 (also known as MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca‑dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12-47 and 46-81) and one high-affinity Ca‑binding site (aa 59-70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP‑2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Item	Value
Long Name	S100 Calcium Binding Protein A8
Entrez Gene IDs	6279 (Human); 20201 (Mouse)
Alternate Names	60B8AG, CAGACP-10, CFAG, CFAGL1Ag, CGLA, Calgranulin A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MA387, MIF, MRP-8, MRP8, MRP8S100 calcium binding protein A8 (calgranulin A), Migration inhibitory factor-related protein 8, NIF, P8, S100 calcium binding protein A8, S100 calcium-binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, Urinary stone protein band A, calgranulin-A, protein S100-A8

Citations for Human S100A8 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human S100A8 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

EGG Sprenkeler et al. (2022), S100A8/A9 Is a Marker for the Release of Neutrophil Extracellular Traps and Induces Neutrophil Activation Cells, 11(2).
PMID: 35053354
Species: Human
Sample Types: Whole Tissue
Applications: IHC

K Asleh et al. (2022), Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes Nature Communications, 13(1):896.
PMID: 35173148
Species: Human
Sample Types: Whole Tissue
Applications: IHC

J Schmidt et al. (2020), Lipid and protein tumor markers for head and neck squamous cell carcinoma identified by imaging mass spectrometry Oncotarget, 11(28):2702-2717.
PMID: 32733643
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

KC Chang et al. (2020), Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma Scientific Reports, 10(1):21342.
PMID: 33288848

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Customer Reviews for Human S100A8 Antibody (1)

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Human S100A8 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Appendix

Species: Human

Verified Customer | Posted 10/25/2021

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Human S100A8 Antibody

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Applications for Human S100A8 Antibody

Scientific Data Examples for Human S100A8 Antibody

Western Blot

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: S100A8

Citations for Human S100A8 Antibody (4)

Customer Reviews for Human S100A8 Antibody (1)

Customer Images

Human S100A8 Antibody

Product Documents for Human S100A8 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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