Human RBX1 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB11499AFP488

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FAB11499AFP488-100UG
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1076128

View all RBX1 Antibodies »

Product Specifications

Immunogen

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL1/RBX1 Complex

Specificity

Detects human RBX1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human RBX1 Alexa Fluor™ Plus 488-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RBX1

RBX1 is a constitutively expressed RING-box protein that interacts with cullins. The human RBX1 gene consists of 5 exons and 4 introns and encodes 108 amino acids with a molecular weight of about 14kda. It is a major component of E3 ubiquitin ligase. A characteristic feature of RBX1 structure is that it contains a RING-H2 finger domain at the carboxyl terminus.  RBX1 is overexpressed in many primary tumors and is associated with overall survival and lymph node metastasis. In humans, RBX1 has high expression in the heart, skeletal muscle, and testis with less expression in the brain, lung, kidney, and placenta.

References

  1. Shao J, Feng Q, Jiang W, Yang Y, Liu Z, Li L, Yang W, Zou Y. E3 ubiquitin ligase RBX1 drives the metastasis of triple negative breast cancer through a FBXO45-TWIST1-dependent degradation mechanism. Aging (Albany NY). 2022 Jul 8;14(13):5493-5510. doi: 10.18632/aging.204163. Epub 2022 Jul 8. PMID: 35802537; PMCID: PMC9320552. 
  2. Wei D, Sun Y. Small RING Finger Proteins RBX1 and RBX2 of SCF E3 Ubiquitin Ligases: The Role in Cancer and as Cancer Targets. Genes Cancer. 2010 Jul;1(7):700-7. doi: 10.1177/1947601910382776. PMID: 21103004; PMCID: PMC2983490.

Long Name

Ring-Box 1

Alternate Names

Ring Box 1, RNF75, ZYP Protein

Entrez Gene IDs

9978 (Human); 56438 (Mouse); 300084 (Rat)

Gene Symbol

RBX1

UniProt

P62877 (Human)

Additional RBX1 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RBX1 Alexa Fluor™ Plus 488-conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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