Detects the endogenous cleaved extracellular domain of human PTP sigma/PTPRS in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human LAR, PTPR beta, PTPR delta, PTPRµ, or PTPR kappa is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PTP sigma/PTPRS Antibody

Detection of Human PTP sigma/PTPRS by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human PTP sigma/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human PTPR mu, PTPR kappa, LAR, PTPR sigma, PTPR delta, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PTP sigma/PTPRS in A431 Cell Line.

PTPs/PTPRS was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human PTPs/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human PTP sigma/PTPRS Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP sigma/PTPRS

Protein Tyrosine Phoshatase Receptor-type sigma (PTPR sigma), also known as PTP sigma, is an integral membrane protein with a non-glycosylated molecular weight of 214 kilodaltons. The extracellular domain, which was used as immunogen, is cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Significant levels of this protein are found in the brain and in endocrine tissues such as the pituitary gland and pancreas. Knockout mice deficient in PTPR sigma have severe neurological deficits and most die shortly after birth.

Long Name

Protein Tyrosine Phosphatase Receptor-type Sigma

Alternate Names

PTPR sigma, PTPRS

Entrez Gene IDs

5802 (Human); 19280 (Mouse)

Gene Symbol

PTPRS

UniProt

NP_570924

Additional PTP sigma/PTPRS Products

Product Documents for Human PTP sigma/PTPRS Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PTP sigma/PTPRS Antibody

For research use only

Citations
Reviews

Human PTP sigma/PTPRS Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3430

Key Product Details

Species Reactivity

Validated:

Cited:

Applications