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Human PTGER2 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB6656

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MAB6656
MAB6656-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 607077

Product Summary for Human PTGER2 Antibody

Immunogen

NS0 mouse myeloma cell line transfected with human PTGER2
Accession # P43116

Specificity

Detects human PTGER2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PTGER2 Antibody

PTGER2 antibody in Human Bladder by Immunohistochemistry (IHC-P).

PTGER2 in Human Bladder.

PTGER2 was detected in immersion fixed paraffin-embedded sections of human bladder using Human PTGER2 Monoclonal Antibody (Catalog # MAB6656) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Strongest staining was localized to umbrella cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human PTGER2 antibody by Western Blot.

Detection of Human PTGER2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human PTGER2 Monoclonal Antibody (Catalog # MAB6656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTGER2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PTGER2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human bladder

Western Blot

2 µg/mL
Sample: MCF‑7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB6656 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTGER2

Prostaglandin E2 receptor (PTGER2) is a 53 kDa member of the G-protein coupled receptor family of proteins. Human PTGER2 is 358 amino acids (aa) in length and has the seven putative transmembrane domains characteristic of G-protein coupled receptors. In addition, there are four potential sites for N-linked glycosylation.  Human PTGER2 shares 86% and 83% aa sequence identity with mouse and rat PTGER2, respectively. PTGER2 is present in the vasculature, the gastrointestinal, tracheobronchial, and reproductive tracts, the kidney, and also in the sphincter, dilator, and ciliary muscles in the eye.  PTGER2 is linked to prostaglandin E2-mediated vasodilation and smooth muscle relaxation. It is also involved in the stimulation of secretion in the stomach and small intestine, the control of intraocular pressure, and the regulation of bone resorption.

Long Name

Prostaglandin E2 Receptor 2, Subtype EP2

Alternate Names

EP2

Entrez Gene IDs

5732 (Human); 19217 (Mouse); 81752 (Rat)

Gene Symbol

PTGER2

UniProt

Additional PTGER2 Products

Product Documents for Human PTGER2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PTGER2 Antibody

For research use only

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