Detects human Integrin beta1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta3, beta4, beta5, beta8, recombinant mouse Integrin beta1, beta2, beta6, or beta7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Porcine/Equine Integrin beta1/CD29 Antibody

Detection of Integrin beta1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (MAB004), open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Detection of Integrin beta1/CD29 in Porcine Mesenchymal Stem Cells by Flow Cytometry.

Porcine mesenchymal stem cells were stained with Mouse Anti-Human/Porcine/Equine Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of Integrin beta1/CD29 in Equine PBMCs by Flow Cytometry.

Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

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Applications for Human/Porcine/Equine Integrin beta1/CD29 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs), porcine mesenchymal stem cells, and equine peripheral blood mononuclear cells (PBMCs)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MG-63 human osteosarcoma cell line and porcine mesenchymal stem cells

Western Blot

1 µg/mL
Sample: Recombinant Human Integrin beta1/CD29 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta1 subunit, also known as VLA-beta chain or CD29, associates with at least ten different Integrin alpha subunits. Within aa 21-728, human Integrin beta1 subunit is 94% aa identical to pig and sheep Integrin beta1 subunits.

Alternate Names

CD29, ITGB1

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Gene Symbol

ITGB1

UniProt

P05556

Additional Integrin beta 1/CD29 Products

Product Documents for Human/Porcine/Equine Integrin beta1/CD29 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Porcine/Equine Integrin beta1/CD29 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Porcine/Equine Integrin beta1/CD29 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB17783

Key Product Details

Species Reactivity

Validated:

Cited:

Applications