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Human PlGF Antibody
Catalog # AB-264-PB | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Summary for Human PlGF Antibody
Immunogen
E. coli-derived recombinant human PlGF (R&D Systems, Catalog # 264-PG)
Ala21-Arg149
Accession # CAA38698
Ala21-Arg149
Accession # CAA38698
Specificity
Detects human PlGF but not VEGF in direct ELISAs and Western blots. This antibody will also recognize the heterodimer of VEGF and PlGF.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human PlGF Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human PlGF (Catalog # 264-PG)
Sample: Recombinant Human PlGF (Catalog # 264-PG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Protein A or G purified
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PlGF
Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. The gene for PlGF has been mapped to chromosome 14. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread, but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to Flt-1, but not to Flk-1/KDR.
Product Documents for Human PlGF Antibody
Product Specific Notices for Human PlGF Antibody
For research use only
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