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Human PlGF Antibody
Catalog # MAB2642 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 358905
Product Summary for Human PlGF Antibody
Immunogen
E.coli-derived recombinant human PLGF
Ala21-Ala149 (predicted)
Accession # P49763
Ala21-Ala149 (predicted)
Accession # P49763
Specificity
Detects human PLGF in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CTGF, rhLDGF, rhPDGF, rhVEGF-C, rhVEGF-D, rhVEGF-B 186, rhVEGF 206, or recombinant mouse PlGF-2 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human PlGF Antibody
PlGF in Human Placenta.
PlGF was detected in immersion fixed paraffin-embedded sections of human placenta using Human PlGF Monoclonal Antibody (Catalog # MAB2642) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PlGF Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta
Sample: Immersion fixed paraffin-embedded sections of human placenta
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 3 publications using MAB2642 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PlGF
Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to VEGF R1/Flt-1 but not to VEGF R2/Flk-1.
Product Documents for Human PlGF Antibody
Product Specific Notices for Human PlGF Antibody
For research use only
Citations for Human PlGF Antibody (3)
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Citations are publications that use Bio-Techne products. Selected citations for Human PlGF Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICCM Ohgushi et al. (2022-06-21), Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model Cell Reports, 2022-039(12):110973.
PMID: 35732132 -
Species: Human
Sample Types: Whole Cells
Applications: IHC(2016-09-23), Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease Sci Rep, 2016-06(0):32382.
PMID: 27660926 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PEl-Tarhouny S et al. (2013-08-13), Placental growth factor and soluble Fms-like tyrosine kinase 1 in diabetic pregnancy: A possible relation to distal villous immaturity. Histol Histopathol, 2013-029(2):259-72.
PMID: 23939615
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