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Human PLA2G16 Antibody

Bio-Techne includes R&D Systems | Catalog # AF6190

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AF6190
AF6190-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Summary for Human PLA2G16 Antibody

Immunogen

E. coli-derived recombinant human PLA2G16
Asp12-Asp132
Accession # P53816

Specificity

Detects human PLA2G16 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PLA2G16 Antibody

Detection of Human PLA2G16 antibody by Western Blot.

Detection of Human PLA2G16 by Western Blot.

Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PLA2G16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6190) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLA2G16 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human PLA2G16 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using AF6190 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLA2G16

PLA2G16 (Group 16 phospholipase A2; also HREV107 and AdPLA) is a Ca++‑dependent 17‑18 kDa member of the H‑rev107 family of molecules. It is expressed intracellularly by white adipocytes, induced by insulin, and generates arachadonic acid from phospholipids. The arachadonic acid is converted to PGE2, which binds the EP3 receptor and decreases cAMP levels. This results in decreased hormone‑sensitive lipase activity and increased adiposity. Human PLA216 is 162 amino acids (aa) in length. It is associated with both membranes and cytosol, and may possess a transmembrane segment between aa 134‑154. The enzymatic activity resides in His23 and Cys113. There is one potential isoform that shows a 20 aa substitution for the first four amino acids. Over aa 12 ‑ 132, human PLA2G16 shares 88% aa identity with mouse PLA2G16.

Long Name

Phospholipase A2, Group XVI

Alternate Names

AdPLA, HRASLS3, HREV107

Entrez Gene IDs

11145 (Human); 225845 (Mouse); 24913 (Rat)

Gene Symbol

PLAAT3

UniProt

Additional PLA2G16 Products

Product Documents for Human PLA2G16 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PLA2G16 Antibody

For research use only

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