Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human PIK3R5 Monoclonal Antibody (Catalog # MAB6388) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PIK3R5 at approximately 97 kDa and the spliced isoform at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human PIK3R5 Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIK3R5

Phosphoinositide 3-kinase regulatory subunit 5 (PIK3R5; also PI3-kinase p101 subunit, Ptdlns-3-kinase p101, and p101-PI3K) is a 97 kDa regulatory subunit of the PI3K gamma complex. It is expressed as a heterodimer with the catalytic subunit PIK3CG/p120. Human PIK3R5 is 880 amino acids (aa) in length. The heterodimerization region is made up of aa 25-101, and aa 653-753 comprise the region for interaction with G beta gamma proteins. A second 55 kDa isoform is formed by the deletion of aa 1-386. Human PIK3R5 is 86% aa identical to mouse PIK3R5. PIK3R5 is highly expressed in leukocytes, followed by spleen, lymph node, thymus, and bone marrow.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 5

Alternate Names

FOAP-2, p101-PI3K

Entrez Gene IDs

23533 (Human); 320207 (Mouse); 497931 (Rat)

Gene Symbol

PIK3R5

UniProt

Q8WYR1 (Human)

Additional PIK3R5 Products

Product Documents for Human PIK3R5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PIK3R5 Antibody

For research use only

Citations
Reviews

Human PIK3R5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6388

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PIK3R5 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PIK3R5 Antibody

Detection of Human PIK3R5 by Western Blot.