Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-VEGFR1/Flt-1 (Y1213) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4170), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-VEGFR1/Flt-1 (Y1213) at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Pervanadate-treated HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

VEGFR1, also known as Flt-1, is a receptor tyrosine kinase that contains seven Ig-like repeats in its extracellular domain, a single transmembrane region, and a kinase domain in the intracellular region. Ligand binding induces autophosphorylation on multiple intracellular tyrosine residues. Y1213 is a major binding site for PI3K, Nck, and SHP-2.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody

For research use only

Citations
Reviews

Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4170

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all VEGFR1/Flt-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody

Detection of Human Phospho-VEGFR1/Flt‑1 (Y1213) by Western Blot.