Human Phospho-FAK (Y397) Antibody
Catalog # MAB4528 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human Phospho-FAK (Y397) Antibody
Immunogen
Accession # Q05397
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Phospho-FAK (Y397) Antibody
Detection of Human Phospho-FAK (Y397) by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-FAK (Y397) Antibody
Western Blot
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate (PV)
Reviewed Applications
Read 1 review rated 4 using MAB4528 in the following applications:
Published Applications
Read 3 publications using MAB4528 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FAK
Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.
Product Documents for Human Phospho-FAK (Y397) Antibody
Product Specific Notices for Human Phospho-FAK (Y397) Antibody
For research use only
Citations for Human Phospho-FAK (Y397) Antibody (3)
Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-FAK (Y397) Antibody include:
-
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotK Wantoch vo et al. (2020-12-03), Insight into Cisplatin-Resistance Signaling of W1 Ovarian Cancer Cells Emerges mTOR and HSP27 as Targets for Sensitization Strategies International Journal of Molecular Sciences, 2020-121(23).
PMID: 33287446 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotJH Pak et al. (2017-03-08), Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin ?4-FAK/Src signaling pathway Mol. Biochem. Parasitol, 2017-00(0).
PMID: 28286026 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotYe F et al. (2013-03-27), Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells. J Virol, 2013-087(11):6326-35.
PMID: 23536671
There are no citations that match your criteria.
Customer Reviews for Human Phospho-FAK (Y397) Antibody (1)
Customer Images
-
Application: ImmunohistochemistrySample Tested: oct embedded section from mouse pancreati tumor and oct embedded section from mouse pancreatic tumorSpecies: MouseVerified Customer | Posted 04/18/2018Mouse pancreatic cancer cells were implanted in C57 BL/6 syngeneic host for 1 month Tumor tissue was fixed in 4% PFA in PBS overnight in cold Then equilibriated in 30% sucrose in PBS in cold overnight Embedded in OCT and sectioned at 10um Stain with antibody for 2h after blocking with Fc recerptor Counterstained with DAPI
There are no reviews that match your criteria.
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.