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Human Phospho-FAK (Y397) Antibody
Catalog # MAB4528 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 820755
Product Summary for Human Phospho-FAK (Y397) Antibody
Immunogen
Phosphopeptide containing the human FAK Y397 site
Accession # Q05397
Accession # Q05397
Specificity
Detects human FAK when phosphorylated at Y397.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Phospho-FAK (Y397) Antibody
Detection of Human Phospho-FAK (Y397) by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-FAK (Y397) Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate (PV)
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate (PV)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB4528 in the following applications:
Published Applications
Read 3 publications using MAB4528 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FAK
Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.
Product Documents for Human Phospho-FAK (Y397) Antibody
Product Specific Notices for Human Phospho-FAK (Y397) Antibody
For research use only
Citations for Human Phospho-FAK (Y397) Antibody (3)
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Citations are publications that use Bio-Techne products. Selected citations for Human Phospho-FAK (Y397) Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotK Wantoch vo et al. (2020-12-03), Insight into Cisplatin-Resistance Signaling of W1 Ovarian Cancer Cells Emerges mTOR and HSP27 as Targets for Sensitization Strategies International Journal of Molecular Sciences, 2020-121(23).
PMID: 33287446 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotJH Pak et al. (2017-03-08), Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin ?4-FAK/Src signaling pathway Mol. Biochem. Parasitol, 2017-00(0).
PMID: 28286026 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotYe F et al. (2013-03-27), Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells. J Virol, 2013-087(11):6326-35.
PMID: 23536671
There are no citations that match your criteria.
Customer Reviews for Human Phospho-FAK (Y397) Antibody (1)
4 out of 5
1 customer
rating
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Application: ImmunohistochemistrySample Tested: oct embedded section from mouse pancreati tumor and oct embedded section from mouse pancreatic tumorSpecies: MouseVerified Customer | Posted 04/18/2018Mouse pancreatic cancer cells were implanted in C57 BL/6 syngeneic host for 1 month Tumor tissue was fixed in 4% PFA in PBS overnight in cold Then equilibriated in 30% sucrose in PBS in cold overnight Embedded in OCT and sectioned at 10um Stain with antibody for 2h after blocking with Fc recerptor Counterstained with DAPI
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