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Human Phospho-FAK (Y397) Antibody

Catalog # MAB4528 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity



Western Blot



Antibody Source

Monoclonal Rat IgG2A Clone # 820755

Product Summary for Human Phospho-FAK (Y397) Antibody


Phosphopeptide containing the human FAK Y397 site
Accession # Q05397


Detects human FAK when phosphorylated at Y397.







Scientific Data Images for Human Phospho-FAK (Y397) Antibody

Detection of Human Phospho-FAK (Y397) antibody by Western Blot.

Detection of Human Phospho-FAK (Y397) by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-FAK (Y397) Antibody

Recommended Usage

Western Blot

0.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate (PV)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB4528 in the following applications:

Published Applications

Read 3 publications using MAB4528 in the following applications:

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant


Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAK

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.

Long Name

Focal adhesion kinase 1

Alternate Names


Entrez Gene IDs

5747 (Human); 14083 (Mouse); 25614 (Rat)

Gene Symbol



Product Documents for Human Phospho-FAK (Y397) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Phospho-FAK (Y397) Antibody

For research use only

Citations for Human Phospho-FAK (Y397) Antibody (3)

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Customer Reviews for Human Phospho-FAK (Y397) Antibody (1)

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  • Human Phospho-FAK (Y397) Antibody
    Name: Mario Shields
    Application: Immunohistochemistry
    Sample Tested: oct embedded section from mouse pancreati tumor and oct embedded section from mouse pancreatic tumor
    Species: Mouse
    Verified Customer | Posted 04/18/2018
    Mouse pancreatic cancer cells were implanted in C57 BL/6 syngeneic host for 1 month Tumor tissue was fixed in 4% PFA in PBS overnight in cold Then equilibriated in 30% sucrose in PBS in cold overnight Embedded in OCT and sectioned at 10um Stain with antibody for 2h after blocking with Fc recerptor Counterstained with DAPI
    Human Phospho-FAK (Y397) Antibody MAB4528

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