Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 5 minutes and 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB1825) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-ERK1 (T202/Y204) and Phospho-ERK2 (T185/Y187) at approximately 43 and 41 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human PDGF‑BB (Catalog # 220-BB) or PMA

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1, ERK-1, ERT2, Extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAPK 1, Microtubule-associated protein 2 kinase, mitogen-activated protein kinase 3, p44erk1, p44-ERK1, p44mapk, p44-MAPK, PRKM3

Additional ERK1/ERK2 Products

Product Documents for Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

For research use only

Citations
Reviews

Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1825

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

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Label

Antibody Source

View all ERK1/ERK2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

Detection of Human and Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot.