Human Phospho-eIF2 alpha (S52) Antibody

Detection of Human Phospho-eIF2 alpha (S52) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes or 20 mJ/cm2 ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-eIF2a (S52) Monoclonal Antibody (Catalog # MAB39971) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-eIF2a (S52) at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-eIF2 alpha (S52) by Western Blot

Analysis of signal transduction during CHIKV infection in the presence of 50 nM silvestrol. (A) HEK 293T cells were seeded in six-well plates and were infected with CHIKV using an MOI of 1, and 50 nM silvestrol was added where indicated. Cells were treated with IFN alpha for 30 min before harvest if indicated, and Western blot analysis of cell lysates was performed. (−) Untreated cells; (+) treated cells. In lanes 3–6, cells were infected with CHIKV. The CHIKV E2 protein, STAT1, eIF2 alpha, and their phosphorylated proteins were detected with specific antibodies and secondary HRP-coupled antibodies, and the ECL detection system (Amersham, Freiburg). Equal loading of each blot was controlled by detection of beta-actin; and, (B) Uninfected HEK293T cells were treated with IFN alpha for 30 min before harvest and either treated with silvestrol for 16 h or left untreated. Western blot analysis of cell lysates was performed and p-STAT1 and STAT1 were detected. STAT1 served as a loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30380742), licensed under a CC-BY license. Not internally tested by R&D Systems.