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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 849159

Product Specifications

Immunogen

Phosphopeptide containing the human eIF2α S52 site
Accession # P05198

Specificity

Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: eIF2 alpha

Eukaryotic translation initiation factor 2 alpha subunit (eIF2 alpha) is a subunit of the eIF2 protein, an important regulator of translation initiation. Phosphorylation of eIF2 alpha on Ser 52 increases the affinity of eIF2 alpha for eIF2B, a guanine nucleotide exchange factor needed for the recycling of eIF2-GDP to eIF2-GTP. Reduction of eIF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all eIF2 alpha kinases.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Entrez Gene IDs

1965 (Human); 13665 (Mouse); 54318 (Rat)

Gene Symbol

EIF2S1

UniProt

Additional eIF2 alpha Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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