HEK293 human embryonic kidney cell line transfected with either (A) human P2X1/P2RX1 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human P2X1/P2RX1 monoclonal antibody (Catalog # MAB10533) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human P2X1/P2RX1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line transfected with Human P2X1/P2RX1 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P2X1/P2RX1

Biologic responses to ATP, ADP, and other extracellular nucleotides are mediated by P2-nucleotide receptors belonging to 2 major classes: G protein-coupled P2Y receptors and nucleotide-gated ion channel P2X receptors. P2RX1 functions as a ligand-gated ion channel with relatively high calcium permeability. Mouse studies suggest that this receptor is essential for normal male reproductive function. The development of selective antagonists for this receptor may provide an effective non-hormonal male contraceptive pill.

Long Name

P2X Purinergic Receptor 1

Alternate Names

P2RX1, P2XMR

Entrez Gene IDs

5023 (Human); 25505 (Rat)

Gene Symbol

P2RX1

UniProt

P51575

Additional P2X1/P2RX1 Products

Product Documents for Human P2X1/P2RX1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human P2X1/P2RX1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human P2X1/P2RX1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10533

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all P2X1/P2RX1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human P2X1/P2RX1 Antibody

Detection of P2X1/P2RX1 in HEK293 Human Cell Line transfected with Human P2X1/P2RX1 and eGFP by Flow Cytometry