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Human Otx2 Antibody Best Seller

Bio-Techne includes R&D Systems | Catalog # AF1979

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AF1979
AF1979-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human Otx2 Antibody

Immunogen

E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243

Specificity

Detects human Otx2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Otx2 Antibody

Detection of Human Otx2 antibody by Western Blot.

Detection of Human Otx2 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Otx2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Otx2 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).

Otx2 in NTera‑2 Human Cell Line.

Otx2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Otx2 antibody in Mouse Embryo by Immunohistochemistry (IHC-P).

Otx2 in Mouse Embryo.

Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Otx2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.)

Simple Western

10 µg/mL
Sample: IMR‑32 human neuroblastoma cell line

Western Blot

1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 9 reviews rated 4.8 using AF1979 in the following applications:

Published Applications

Read 158 publications using AF1979 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Otx2

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

References

  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Entrez Gene IDs

5015 (Human)

Gene Symbol

OTX2

UniProt

Additional Otx2 Products

Product Documents for Human Otx2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Otx2 Antibody

For research use only

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