Human Otx2 Antibody

Name: Human Otx2 Antibody
Brand: R&D Systems
SKU: AF1979
Rating: 5 (6 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1979

Citations (74)

(6)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human Otx2 Met1-Leu289 Accession # P32243
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Otx2 in direct ELISAs and Western blots.

View Full Technical Details for Human Otx2 Antibody

Applications for Human Otx2 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	1-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Otx2 Antibody

	Western Blot Detection of Human Otx2 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Otx2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry Otx2 in NTera‑2 Human Cell Line. Otx2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry Otx2 in Mouse Embryo. Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Simple Western Detection of Human Otx2 by Simple Western^TM. Simple Western lane view shows lysates of IMR-32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Otx2

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

Item	Value
References	Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.
Long Name	Orthodenticle Homolog 2
Entrez Gene IDs	5015 (Human)
Alternate Names	MCOPS5, MGC45000, Orthodenticle homolog 2, Otx2, homeobox protein OTX2, orthodenticle homeobox 2, orthodenticle homolog 2 (Drosophila)

Citations for Human Otx2 Antibody (74)

Showing 1 - 10 of 74 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Otx2 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

S Touhami et al. (2022-01-01), Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently International Journal of Molecular Sciences, 23(2).
PMID: 35054863
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

P Nouri et al. (2022-01-01), Generation of a NES-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene Editing Cells, 11(2).
PMID: 35053384
Species: Human
Sample Types: Transfected Whole Cells
Applications: ICC

HM Lee et al. (2022-01-01), Generation of alphaMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting Stem Cell Research, 61(0):102779.
PMID: 35427904
Species: Human
Sample Types: Whole Cells
Applications: ICC

A Manhas et al. (2022-01-01), Generation of two iPSC lines from hypertrophic cardiomyopathy patients carrying MYBPC3 and PRKAG2 variants Stem Cell Research, 61(0):102774.
PMID: 35413566
Species: Human
Sample Types: Whole Cells
Applications: ICC

IC Han et al. (2022-01-01), Biocompatibility of Human Induced Pluripotent Stem Cell-Derived Retinal Progenitor Cell Grafts in Immunocompromised Rats Cell Transplantation, 31(0):9636897221104.
PMID: 35758274
Species: Human
Sample Types: Whole Cells
Applications: ICC

RR Khadim et al. (2022-01-01), Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs International Journal of Molecular Sciences, 23(13).
PMID: 35806277
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

M Alonzo et al. (2022-01-01), Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS) Stem Cell Research, 64(0):102892.
PMID: 35987121
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Contreras et al. (2022-01-01), Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS) Stem Cell Research, 64(0):102893.
PMID: 35987120
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Liu et al. (2022-01-01), Generation of a human induced pluripotent stem cell line carrying the TYR c.575C>A (p.Ser192Tyr) and c.1205G>A (p.Arg402Gln) variants in homozygous state using CRISPR-Cas9 genome editing Stem Cell Research, 64(0):102880.
PMID: 35933957
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Lotila et al. (2022-01-01), Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient Oncogene, 63(0):102865.
PMID: 35843021
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC/IF

E Boobalan et al. (2022-01-01), Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure Investigative Ophthalmology Visual Science, 2022;63(12):5.
PMID: 36326727
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

M Langouët et al. (2022-01-01), Mutations in BCOR, a co-repressor of CRX/OTX2, are associated with early-onset retinal degeneration Science Advances, 8(36):eabh2868.
PMID: 36070393
Species: Mouse
Sample Types: Embryo
Applications: IHC

MA Molè et al. (2021-01-01), Integrin &beta1 coordinates survival and morphogenesis of the embryonic lineage upon implantation and pluripotency transition Cell Reports, 34(10):108834.
PMID: 33691117
Species: Human
Sample Types: Whole Cells
Applications: ICC

G Iannello et al. (2021-01-01), Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity Stem Cell Research, 54(0):102432.
PMID: 34214899
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

L Liu et al. (2021-01-01), Generation of two heterozygous MYBPC3 mutation-carrying human iPSC lines, SCVIi001-A and SCVIi002-A, for modeling hypertrophic cardiomyopathy Stem Cell Research, 53(0):102279.
PMID: 33743363
Species: Human
Sample Types: Embryo
Applications: ICC

A Yanagida et al. (2021-01-01), Naive stem cell blastocyst model captures human embryo lineage segregation Cell Stem Cell, 28(6):1016-1022.e4.
PMID: 33957081
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

S Lim et al. (2021-01-01), mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent Mï¿½ller glia Elife, 10(0).
PMID: 34677125
Species: Medaka
Sample Types: Organoid
Applications: IHC

L Zilova et al. (2021-01-01), Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development Elife, 10(0).
PMID: 34252023
Species: Human
Sample Types: Embryo
Applications: IHC

D Meisterman et al. (2021-01-01), Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification Cell Stem Cell, 0(0).
PMID: 34004179
Species: Human
Sample Types: Whole Cells
Applications: ICC

F Nilsson et al. (2021-01-01), Single-Cell Profiling of Coding and Noncoding Genes in Human Dopamine Neuron Differentiation Cells, 10(1).
PMID: 33445654
Species: Mouse
Sample Types: Embryo
Applications: IHC

A Weberling et al. (2021-01-01), Trophectoderm mechanics direct epiblast shape upon embryo implantation Cell Reports, 34(3):108655.
PMID: 33472064
Species: Mouse
Sample Types: Organoid
Applications: ICC

J Langkabel et al. (2021-01-01), Induction of Rosette-to-Lumen stage embryoids using reprogramming paradigms in ESCs Nature Communications, 12(1):7322.
PMID: 34916498
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

N Belbachir et al. (2021-01-01), Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations Stem Cell Research, 57(0):102605.
PMID: 34856468
Species: Human
Sample Types: Whole Cells
Applications: ICC

P Aldrin-Kir et al. (2021-01-01), A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants Stem Cell Reports, 17(1):159-172.
PMID: 34971563
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

C Lee et al. (2021-01-01), Generation of three iPSC lines from dilated cardiomyopathy patients carrying a pathogenic LMNA variant Stem Cell Research, 59(0):102638.
PMID: 34954454
Species: Human
Sample Types: Whole Tissue
Applications: IHC

G Iannello et al. (2021-01-01), Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL Stem Cell Research, 59(0):102635.
PMID: 35074682
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

X Wang et al. (2021-01-01), Formative pluripotent stem cells show features of epiblast cells poised for gastrulation Cell Research, 0(0).
PMID: 33608671
Species: Human

Applications: IF

N Micali et al. (2020-01-01), Variation of Human Neural Stem Cells Generating Organizer States Inï¿½Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates Cell Rep, 31(5):107599.
PMID: 32375049
Species: Pan troglodytes (Chimpanzee)
Sample Types: Whole Cells
Applications: ICC

R Kitajima et al. (2020-01-01), Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells Stem Cell Res, 44(0):101749.
PMID: 32151953
Species: Rat
Sample Types: Cell Culture Lysates
Applications: Western Blot

M Bistoletti et al. (2020-01-01), Homeoprotein OTX1 and OTX2 involvement in rat myenteric neuron adaptation after DNBS-induced colitis PeerJ, 8(0):e8442.
PMID: 32095330
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

CW Gantner et al. (2020-01-01), Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease Cell Stem Cell, 0(0).
PMID: 32059808
Species: Human
Sample Types: Whole Tissue
Applications: IHC

F Jacob et al. (2020-01-01), Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium Cell Stem Cell, 0(0).
PMID: 33010822
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Malankhano et al. (2020-01-01), A Human Induced Pluripotent Stem Cell-Derived Isogenic Model of Huntington's Disease Based on Neuronal Cells Has Several Relevant Phenotypic Abnormalities J Pers Med, 10(4).
PMID: 33182269
Species: Mouse
Sample Types: Whole Cells
Applications: IHC

H De Belly et al. (2020-01-01), Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate Cell Stem Cell, 0(0).
PMID: 33217323
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

LTP Khoa et al. (2020-01-01), Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid Oxidation Cell Stem Cell, 0(0).
PMID: 32610040
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

LS VandenBosc et al. (2020-01-01), Developmental changes in the accessible chromatin, transcriptome and Ascl1-binding correlate with the loss in Mï¿½ller Glial regenerative potential Sci Rep, 10(1):13615.
PMID: 32788677
Species: Human
Sample Types: Whole Cells
Applications: ICC

PA Barbuti et al. (2020-01-01), Generation of two iPS cell lines (HIHDNDi001-A and HIHDNDi001-B) from a Parkinsonï¿½s disease patient carrying the heterozygous p.A30P mutation in SNCA Stem Cell Res, 48(0):101951.
PMID: 32798915
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

C Kyprianou et al. (2020-01-01), Basement membrane remodelling regulates mouse embryogenesis Nature, 582(7811):253-258.
PMID: 32523119
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Riera et al. (2019-01-01), Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene Stem Cell Res, 35(0):101386.
PMID: 30685615
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Riera et al. (2019-01-01), Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations Stem Cell Res, 35(0):101385.
PMID: 30685614
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Y Chen et al. (2019-01-01), Photoreceptor degeneration in microphthalmia (Mitf) mice: partial rescue by pigment epithelium-derived factor Dis Model Mech, 12(1).
PMID: 30651300
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

N Ruiz-Reig et al. (2019-01-01), Developmental Requirement of Homeoprotein Otx2 for Specific Habenulo-Interpeduncular Subcircuits J. Neurosci., 39(6):1005-1019.
PMID: 30593496
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Riera et al. (2019-01-01), Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations Stem Cell Res, 36(0):101389.
PMID: 30798147
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Domingo-Pr et al. (2019-01-01), Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene Stem Cell Res, 38(0):101468.
PMID: 31146251
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Domingo-Pr et al. (2019-01-01), Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene Stem Cell Res, 40(0):101569.
PMID: 31520890
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Domingo-Pr et al. (2019-01-01), Generation of Best disease-derived induced pluripotent stem cell line (FRIMOi006-A) carrying a novel dominant mutation in BEST1 gene Stem Cell Res, 40(0):101570.
PMID: 31518904
Species: Human
Sample Types: Whole Cells
Applications: ICC

MT De Angelis et al. (2019-01-01), Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus J. Cell. Mol. Med., 0(0).
PMID: 31536674
Species: Human
Sample Types: Whole Cells
Applications: ICC

SH Chung et al. (2019-01-01), Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway Front Cell Neurosci, 13(0):527.
PMID: 31849614
Species: Human
Sample Types: Whole Cells
Applications: ICC

C Lu et al. (2019-01-01), Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A) Stem Cell Res, 41(0):101643.
PMID: 31707212
Species: Human
Sample Types: Whole Cells
Applications: ICC

AJ Duarte et al. (2019-01-01), Induced pluripotent stem cell line (INSAi001-A) from a Gaucher disease type 3 patient compound heterozygote for mutations in the GBA1 gene Stem Cell Res, 41(0):101595.
PMID: 31678773
Species: Human
Sample Types: Whole Cells
Applications: ICC

JY Kim et al. (2018-01-01), Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells Exp. Mol. Med., 50(4):24.
PMID: 29650976
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

SE Harrison et al. (2018-01-01), In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells Nat Protoc, 0(0).
PMID: 29988106
Species: Human
Sample Types: Whole Cells
Applications: ICC

AL Gagne et al. (2018-01-01), Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL Stem Cell Res, 31(0):157-160.
PMID: 30096712
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

AD Senft et al. (2018-01-01), Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming Cell Rep, 24(8):1977-1985.e7.
PMID: 30134160
Species: Human
Sample Types: Whole Cells
Applications: Differentiation, Differentiation

S Kaur et al. (2018-01-01), Small non-coding RNA landscape of extracellular vesicles from human stem cells Sci Rep, 8(1):15503.
PMID: 30341351
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

R Diacou et al. (2018-01-01), Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation Cell Rep, 25(9):2510-2523.e4.
PMID: 30485816
Species: Human
Sample Types: Whole Cells
Applications: ICC

KE Worley et al. (2018-01-01), Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation Stem Cells Int, 2018(0):1848605.
PMID: 30627170
Species: Human
Sample Types: Whole Cells
Applications: ICC

JA Maguire et al. (2018-01-01), Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene Stem Cell Res, 34(0):101361.
PMID: 30611021
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

S El Nagar et al. (2018-01-01), A new genetically engineered mouse model of choroid plexus carcinoma Biochem. Biophys. Res. Commun., 0(0).
PMID: 29339161
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

X Tang et al. (2018-01-01), Tau Deficiency Down-Regulated Transcription Factor Orthodenticle Homeobox 2 Expression in the Dopaminergic Neurons in Ventral Tegmental Area and Caused No Obvious Motor Deficits in Mice Neuroscience, 0(0).
PMID: 29337233
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

PA Nakamura et al. (2017-01-01), Small molecule Photoregulin3 prevents retinal degeneration in the RhoP23H mouse model of retinitis pigmentosa Elife, 6(0).
PMID: 29148976
Species: Chicken
Sample Types: Whole Tissue
Applications: IHC

L Todd et al. (2017-01-01), Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of Mï¿½ller Glia-Derived Progenitor Cells in the Avian Retina Stem Cells, 0(0).
PMID: 29193451
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

(2016-01-01), Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-? Aging Cell, 0(0).
PMID: 27660103
Species: Human
Sample Types: Whole Cells
Applications: ICC

Edri et al. (2015-01-01), Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun, 6(0):6500.
PMID: 25799239
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Wu Q et al. (2015-01-01), SMAD2 and p38 signaling pathways act in concert to determine XY primordial germ cell fate in mice. Development, 142(3):575-86.
PMID: 25605784
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Zou M et al. (2015-01-01), Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn, 244(1):86-97.
PMID: 25179941
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Di Giovannantonio et al. (2014-01-01), Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development, 141(2):377-88.
PMID: 24335253
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Stott et al. (2013-01-01), Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages. J Neurosci, 33(18):8022-34.
PMID: 23637192
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Rijlaarsdam MA et al. (2011-01-01), Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours. Br. J. Cancer, 105(6):854-63.
PMID: 21847120
Species: Human
Sample Types: Whole Cells
Applications: ICC

Zeng H et al. (2010-01-01), Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells. PLoS ONE, 5(7):e11853.
PMID: 20686615
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Di Salvio M et al. (2010-01-01), Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP. Nat. Neurosci., 13(12):1481-8.
PMID: 21057506
Species: Human
Sample Types: Whole Cells
Applications: ICC

Gil JE et al. (2009-01-01), Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett., 583(3):561-7.
PMID: 19162023
Species: Human
Sample Types: Whole Cells
Applications: ICC

Pankratz MT et al. (2007-01-01), Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage. Stem Cells, 25(6):1511-20.
PMID: 17332508
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Baulier E et al. (), Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143. Stem Cell Re33(0):274-277.
PMID: 30513407

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Showing 1 - 10 of 74 citations Showing All

Customer Reviews for Human Otx2 Antibody (6)

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6 customer ratings

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Human Otx2 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Brain,neuron cells

Species: Human

Verified Customer | Posted 07/12/2021
Human Otx2 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Adult brain

Species: Human

Verified Customer | Posted 06/29/2021
Human Otx2 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Fetal brain

Species: Human

Verified Customer | Posted 12/21/2020
Human Otx2 Antibody

Name: Alina Filatova

Application: ICC/IF

Sample Tested: Embryonic brain

Species: Mouse

Verified Customer | Posted 10/04/2018

This antibody works very well at 1:1000 in immunofluorescence staining of mouse paraffin-embedded sections. Epitope retrieval: Citrate buffer.
Human Otx2 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Undifferentiated neural progenitor cells

Species: Human

Verified Customer | Posted 08/22/2016

ICC on hPSC derived neurl progenitors
Human Otx2 Antibody

Name: Isis Trujillo

Application: IHC

Sample Tested: Embryonic retina

Species: Mouse

Verified Customer | Posted 06/29/2016

Otx2 staining on frozen mouse embryonic day 17 eyes sections using AF1979 The sections were blocked using 0.01% Triton-X, 2% donkey serum in 1X PBS 1 hour at room temperature. OTX2 was diluted 1:500 using blocking buffer and incubated overnight at 4°C. The secondary antibody used was donkey ant goat conjugated to Alexa Fluor 488 (1:2000).

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Human Otx2 Antibody

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Scientific Data Examples for Human Otx2 Antibody

Western Blot

Immunocytochemistry

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Formulation, Preparation and Storage

Reconstitution Calculator

Background: Otx2

Citations for Human Otx2 Antibody (74)

Customer Reviews for Human Otx2 Antibody (6)

Customer Images

Human Otx2 Antibody

Human Otx2 Antibody

Human Otx2 Antibody

Human Otx2 Antibody

Human Otx2 Antibody

Human Otx2 Antibody

Product Documents for Human Otx2 Antibody

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