Human OLT-2/TARM1 Alexa Fluor® 700-conjugated Antibody

Name: Human OLT-2/TARM1 Alexa Fluor® 700-conjugated Antibody
Brand: R&D Systems
SKU: FAB10059N
Price: 370 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB10059N

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Recombinant Monoclonal Antibody.

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Key Product Details

Species Reactivity:	Human
Immunogen:	Chinese hamster ovary cell line CHO-derived recombinant human OLT-2/TARM-1 Met1-Val235 Accession # B6A8C7
Applications:	Flow Cytometry
Label:	Alexa Fluor 700 (Excitation=675-700 nm, Emission=723 nm)
Source:	Recombinant Monoclonal Rabbit IgG Clone # 2564C
Specificity:	Detects human OLT-2/TARM-1 in direct ELISAs.

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Applications

	Recommended Concentration	Sample
Flow Cytometry	0.25-1 µg/10⁶ cells	Peripheral blood granulocytes or U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: OLT-2/TARM1

T cell interacting, activating receptor on myeloid cells-1 (TARM-1), also known as OSCAR-like transcript-2 protein (OLT-2) is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse OLT-2/TARM-1 contains a 242 aa long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of human OLT-2/TARM-1 shares 48% and 49% amino acid (aa) identity with mouse and rat OLT-2/TARM-1 respectively. OLT-2/TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12.OLT-2/TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. OLT-2/TARM-1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. OLT-2/TARM-1 Fc fusion protein inhibits anti-CD3 induced CD4⁺ cell activation, suggesting that OLT-2/TARM-1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

Item	Value
References	Radjabova, V., et al. (2015) J Immunol. 195:3149.
Long Name	T-cell-interacting Activating Receptor on Myeloid Cells 1
Entrez Gene IDs	441864 (Human); 245126 (Mouse); 499065 (Rat)
Alternate Names	OLT-2/TARM1, OLT2, OSCAR-Like Transcript-2 Protein, T Cell-Interacting, Activating Receptor On Myeloid Cells, T Cell-Interacting, Activating Receptor On Myeloid Cells 1, T-Cell-Interacting, Activating Receptor On Myeloid Cells Protein 1, TARM1

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human OLT-2/TARM1 Alexa Fluor® 700-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Background: OLT-2/TARM1

Product Specific Notices

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