Human OLT-2/TARM1 Antibody

Name: Human OLT-2/TARM1 Antibody
Brand: R&D Systems
SKU: MAB10059
Price: 134 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB10059

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

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Key Product Details

Species Reactivity:	Human
Immunogen:	Chinese hamster ovary cell line CHO-derived recombinant human OLT-2/TARM-1 Met1-Val235 Accession # B6A8C7
Applications:	CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 2564C
Specificity:	Detects human OLT-2/TARM-1 in direct ELISAs.

View Full Technical Details for Human OLT-2/TARM1 Antibody

Applications for Human OLT-2/TARM1 Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	Peripheral blood granulocytes or U937 human histiocytic lymphoma cell line
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human OLT-2/TARM1 Antibody

	Flow Cytometry Detection of OLT-2/TARM1 in Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rabbit anti-Human OLT-2/TARM1 Monoclonal Antibody (Catalog # MAB10059, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Phycoerythrin-conjugated Secondary Antibody (F0110). Staining was performed using our Staining Membrane-Associated Proteins protocol.
	Flow Cytometry Detection of OLT-2/TARM1 on U937 Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Rabbit anti-Human OLT-2/TARM1 Monoclonal Antibody (MAB10059, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Phycoerythrin-conjugated Secondary Antibody (F0110). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: OLT-2/TARM1

T cell interacting, activating receptor on myeloid cells-1 (TARM-1), also known as OSCAR-like transcript-2 protein (OLT-2) is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse OLT-2/TARM-1 contains a 242 aa long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of human OLT-2/TARM-1 shares 48% and 49% amino acid (aa) identity with mouse and rat OLT-2/TARM-1 respectively. OLT-2/TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12.OLT-2/TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. OLT-2/TARM-1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. OLT-2/TARM-1 Fc fusion protein inhibits anti-CD3 induced CD4⁺ cell activation, suggesting that OLT-2/TARM-1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

Item	Value
References	Radjabova, V., et al. (2015) J Immunol. 195:3149.
Long Name	T-cell-interacting Activating Receptor on Myeloid Cells 1
Entrez Gene IDs	441864 (Human); 245126 (Mouse); 499065 (Rat)
Alternate Names	OLT-2/TARM1, OLT2, OSCAR-Like Transcript-2 Protein, T Cell-Interacting, Activating Receptor On Myeloid Cells, T Cell-Interacting, Activating Receptor On Myeloid Cells 1, T-Cell-Interacting, Activating Receptor On Myeloid Cells Protein 1, TARM1

Citations for Human OLT-2/TARM1 Antibody

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Human OLT-2/TARM1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human OLT-2/TARM1 Antibody

Scientific Data Examples for Human OLT-2/TARM1 Antibody

Flow Cytometry

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: OLT-2/TARM1

Citations for Human OLT-2/TARM1 Antibody

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