Human Notch-1 PE-conjugated Antibody

Name: Human Notch-1 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB5317P
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB5317P

Citations (4)

(1)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Notch-1 Ala19-Gln526 Accession # P46531
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 527425
Specificity:	Detects human Notch-1.

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Applications for Human Notch-1 PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Notch-1 PE-conjugated Antibody

	Flow Cytometry Detection of Notch‑1 in U2OS Human Cell Line by Flow Cytometry. U2OS human osteosarcoma cell line was stained with Mouse Anti-Human Notch-1 PE-conjugated Monoclonal Antibody (Catalog # FAB5317P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Notch-1

Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1‑3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2‑4). Notch can specify binary cell fates; for example, promoting T‑ over B‑cell development from a common precursor (2). More than 50% of human T-lineage Acute Lymphoblastic Leukemia (T‑ALL) have activating mutations of Notch1 (1, 5). Human Notch-1 is synthesized as a 2556 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1718 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain containing six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11^th and 12^th EGF-like repeats bind ligands including Jagged and Delta-like families in humans (6). O-fucosylation by Fringe family members at a site within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta-like ligand interactions (7). Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, forming a heterodimer through interaction of a hydrophobic area C-terminal to the LNR on the 1647 aa ligand-binding extracellular region with the 891 aa transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM-17) and the presenilin-dependent gamma -Secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (10). Human Notch-1 ECD aa 19 - 526, including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat, 89% with canine, and 79% with chicken Notch-1. This region also exhibits 60% aa identity with human Notch-2 and Notch-3.

Item	Value
References	Ellisen, L. W. et al. (1991) Cell 66:649. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709. Androutsellis-Theotokis, A. et al. (2006) Nature 442:823. Weng, A. P. et al. (2004) Science 306:269. Hambleton, S. et al. (2004) Structure 12:2173. Yang, L. et al. (2005) Mol. Biol. Cell 16:927. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
Entrez Gene IDs	4851 (Human); 25496 (Rat)
Alternate Names	EC 2.1.2.11, EC 3.4.21.68, Notch (Drosophila) homolog 1 (translocation-associated), Notch homolog 1, translocation-associated, Notch-1, Notch1, TAN1, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1, hN1, notch 1Notch homolog 1, translocation-associated (Drosophila)

Citations for Human Notch-1 PE-conjugated Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Notch-1 PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

YV Teo et al. (2019), Notch Signaling Mediates Secondary Senescence Cell Rep, 27(4):997-1007.e5.
PMID: 31018144
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

A Al-Attar et al. (2018), Activation of Notch-1 in oral epithelial cells by P. gingivalis triggers the expression of the antimicrobial protein PLA2-IIA Mucosal Immunol, 11(4):1047-1059.
PMID: 29515164
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

S Baldoni et al. (2018), Bepridil exhibits anti-leukemic activity associated with NOTCH1 pathway inhibition in chronic lymphocytic leukemia Int. J. Cancer, 0(0).
PMID: 29508386
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Piggott K et al. (2012), Blocking the NOTCH pathway inhibits vascular inflammation in large-vessel vasculitis. Circulation, 123(3):309-18.
PMID: 21220737

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Customer Reviews for Human Notch-1 PE-conjugated Antibody (1)

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Human Notch-1 PE-conjugated Antibody

Name: Ahmad Al-Attar

Application: Flow

Sample Tested: Human primary gingival epithelial cells (TIGK)

Species: Human

Verified Customer | Posted 06/02/2016

So simple! Add the antibody (or its isotype control) to the cells (5uL for 1x10^6 cells), incubate for 30 minutes, wash and read!

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Product Documents for Human Notch-1 PE-conjugated Antibody

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Human Notch-1 PE-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human Notch-1 PE-conjugated Antibody

Scientific Data Examples for Human Notch-1 PE-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: Notch-1

Citations for Human Notch-1 PE-conjugated Antibody (4)

Customer Reviews for Human Notch-1 PE-conjugated Antibody (1)

Customer Images

Human Notch-1 PE-conjugated Antibody

Product Documents for Human Notch-1 PE-conjugated Antibody

Product Datasheets

COA

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