Human Notch-1 Intracellular Domain Antibody
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity: | Human |
Immunogen: |
E. coli-derived recombinant human Notch‑1 Gly2428-Lys2556 Accession # P46531 |
Applications: | Chromatin Immunoprecipitation, CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot |
Label: | Unconjugated |
Source: | Polyclonal Sheep IgG |
Specificity: |
Detects the intracellular domain (ICD) of human Notch-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Notch-2 ICD, rhNotch-3 ICD, and rhNotch-4 ICD is observed. |
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Citations for Human Notch-1 Intracellular Domain Antibody (3)
Citations are publications that use Bio-Techne products. Selected citations for Human Notch-1 Intracellular Domain Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotSC Hrstka et al. (2017), NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis Stem Cells, 0(0).
PMID: 28142228 -
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot(2016), A dual molecular analogue tuner for dissecting protein function in mammalian cells Nat Commun, 7(0):11742.
PMID: 27230261 -
Gogoi D et al. (2014), Involvement of Notch in activation and effector functions of gammadelta T cells. J Immunol, 192(5):2054-62.
PMID: 24489102
There are no citations that match your criteria.
Customer Reviews for Human Notch-1 Intracellular Domain Antibody (4)
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Application: ICC/IFSample Tested: E12.5 mouse embryo fixed in 4% PFASpecies: MouseVerified Customer | Posted 06/23/2021Fixed 4% PFA overnight.Blocked with 1% BSAPrimary antibody dilution - 1:20Secondary antibody - Invitrogen Alexa Fluor 488Secondary antibody dilution - 1:1000Stained on an E12.5 mouse right ventricle heart section
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Application: WBSample Tested: iPS2 human induced pluripotent stem cellsSpecies: HumanVerified Customer | Posted 01/25/2018
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Application: ICC/IFSample Tested: Bladder cancer tissue,Bladder tissueSpecies: HumanVerified Customer | Posted 12/10/2017
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Application: WBSample Tested: Breast cancer cellsSpecies: HumanVerified Customer | Posted 03/01/2017Expression of cleaved Notch-1 (Intracellular Domain) in mammary tumor cell lines. Dilution: 1:200 in PBS with 5% BSA. Secondary Ab: anti-Sheep IgG 1:5,000.
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