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Human Notch-1 Intracellular Domain Antibody

R&D Systems, Inc. a Bio-Techne Brand

Detection of Human Notch-1.
(3)
Notch‑1 antibody in Saos-2 Human Cell Line by Immunocytochemistry (ICC).
Detection of Notch‑1-regulated Genes antibody by Chromatin Immunoprecipitation.
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AF3647
AF3647-SP
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Key Product Details

Species Reactivity: Human
Immunogen: E. coli-derived recombinant human Notch‑1
Gly2428-Lys2556
Accession # P46531
Applications: Chromatin Immunoprecipitation, CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot
Label: Unconjugated
Source: Polyclonal Sheep IgG
Specificity:

Detects the intracellular domain (ICD) of human Notch-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots,  less than 1% cross-reactivity with recombinant human (rh) Notch-2 ICD, rhNotch-3 ICD, and rhNotch-4 ICD is observed.

Citations for Human Notch-1 Intracellular Domain Antibody (3)

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Customer Reviews for Human Notch-1 Intracellular Domain Antibody (4)

3.8 out of 5
4 customer ratings
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  • Human Notch-1 Intracellular Domain Antibody
    Name: Anonymous
    Application: ICC/IF
    Sample Tested: E12.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 06/23/2021
    Fixed 4% PFA overnight.Blocked with 1% BSAPrimary antibody dilution - 1:20Secondary antibody - Invitrogen Alexa Fluor 488Secondary antibody dilution - 1:1000Stained on an E12.5 mouse right ventricle heart section
    Human Notch-1 Intracellular Domain Antibody Immunocytochemistry/Immunofluorescence AF3647
  • Human Notch-1 Intracellular Domain Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 01/25/2018
    Human Notch-1 Intracellular Domain Antibody Western Blot AF3647
  • Human Notch-1 Intracellular Domain Antibody
    Name: Anonymous
    Application: ICC/IF
    Sample Tested: Bladder cancer tissue,Bladder tissue
    Species: Human
    Verified Customer | Posted 12/10/2017
    Human Notch-1 Intracellular Domain Antibody Immunocytochemistry/Immunofluorescence AF3647
  • Human Notch-1 Intracellular Domain Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 03/01/2017
    Expression of cleaved Notch-1 (Intracellular Domain) in mammary tumor cell lines. Dilution: 1:200 in PBS with 5% BSA. Secondary Ab: anti-Sheep IgG 1:5,000.
    Human Notch-1 Intracellular Domain Antibody Western Blot AF3647

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Product Documents for Human Notch-1 Intracellular Domain Antibody

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