Human NG2/MCSP Antibody

NG2/MCSP in Human Melanoma via seqIF™ staining on COMET™

NG2/MCSP was detected in immersion fixed paraffin-embedded sections of human Melanoma using Mouse Anti-Human NG2/MCSP, Monoclonal Antibody (Catalog # MAB11767) at 15 μg/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Human NG2/MCSP by Western Blot.

Western Blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB11767) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immersion fixed paraffin-embedded sections of human melanoma

NG2/MCSP was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB11767) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.