Key Product Details
Product Summary for Human Nanog Antibody
Accession # ABZ92376
Scientific Data Images for Human Nanog Antibody
Detection of Human Nanog by Western Blot.Western blot shows lysates of BG01V human embryonic stem cells and Tera-2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.
Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation.BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR.
Nanog in BG01V Human Stem Cells.Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Applications for Human Nanog Antibody
Chromatin Immunoprecipitation (ChIP)
Sample: BG01V human embryonic stem cell chromatin, for detection see our available Human Nanog Primer Pair (Catalog # RDP-320-025)
Sample: Immersion fixed BG01V human embryonic stem cells and immersion fixed ADLF1 and FAB2 induced pluripotent stem cell lines
Sample: Immersion fixed paraffin-embedded sections of human testicular cancer, immersion fixed paraffin-embedded sections of normal human testis, and immersion fixed frozen sections of 13 d.p.c. mouse embryos
Sample: BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line
Read 11 reviews rated 4 using AF1997 in the following applications:
- Immunocytochemistry (1 Review)
- Immunocytochemistry/Immunofluorescence (3 Reviews)
- Immunofluorescence (2 Reviews)
- Western Blot (5 Reviews)
Read 207 publications using AF1997 in the following applications:
- ChIP (3 Publications)
- ChIP-Seq (2 Publications)
- Differentiation (1 Publication)
- Flow Cytometry (27 Publications)
- ICC (156 Publications)
- ICC/IF (1 Publication)
- IF (1 Publication)
- IHC (19 Publications)
- IHC-Fr (7 Publications)
- IHC-P (1 Publication)
- Immunoprecipitation (3 Publications)
- Western Blot (18 Publications)
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Mitsui, K. et al. (2003) Cell 11:631.
- Chambers, I. et al. (2003) Cell 113:643.
- Hart, A.H. et al. (2004) Dev. Dyn. 230:187.
Product Specific Notices for Human Nanog Antibody
For research use only