Skip to main content

Human Nanog Antibody Best Seller

Catalog # AF1997 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
AF1997
AF1997-SP

Key Product Details

Species Reactivity

Human

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human Nanog Antibody

Immunogen

E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376

Specificity

Detects human Nanog in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Nanog Antibody

Detection of Human Nanog antibody by Western Blot.

Detection of Human Nanog by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells and Tera-2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.
Detection of Nanog-regulated Genes antibody by Chromatin Immunoprecipitation.

Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation.

BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR.
Nanog antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

Nanog in BG01V Human Stem Cells.

Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Nanog Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 106 cells
Sample: BG01V human embryonic stem cell chromatin, for detection see our available Human Nanog Primer Pair (Catalog # RDP-320-025)

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and immersion fixed ADLF1 and FAB2 induced pluripotent stem cell lines

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testicular cancer, immersion fixed paraffin-embedded sections of normal human testis, and immersion fixed frozen sections of 13 d.p.c. mouse embryos

Simple Western

Western Blot

1 µg/mL
Sample: BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 11 reviews rated 4 using AF1997 in the following applications:

Published Applications

Read 207 publications using AF1997 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).

References

  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Long Name

Nanog Homeobox

Alternate Names

Nanog, FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Entrez Gene IDs

79923 (Human); 71950 (Mouse)

Gene Symbol

NANOG

UniProt

Product Documents for Human Nanog Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Nanog Antibody

For research use only

Citations for Human Nanog Antibody (207)

Showing  1 - 10 of 207 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human Nanog Antibody include:

There are no citations that match your criteria.

Showing  1 - 10 of 207 publications Showing All

Customer Reviews for Human Nanog Antibody (11)

4 out of 5
11 customer ratings
5 stars
18%
4 stars
64%
3 stars
18%
2 stars
0%
1 star
0%

Customer Images


Showing  1 - 5 of 11 reviews Showing All
Filter By:
  • Human Nanog Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 10/24/2023
    Nanog IF staining was performed on iPSCs.
    Human Nanog Antibody AF1997
  • Human Nanog Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF7
    Species: Human
    Verified Customer | Posted 12/02/2018
    Human Nanog Antibody AF1997
  • Human Nanog Antibody
    Name: Leslie Priddy
    Application: Western Blot
    Sample Tested: Tumor cell lyastes
    Species: Human
    Verified Customer | Posted 04/13/2018
  • Human Nanog Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryonic stem cells
    Species: Human
    Verified Customer | Posted 11/23/2017
    Human Nanog Antibody AF1997
  • Human Nanog Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 7TD1 mouse hybridoma cell line
    Species: Mouse
    Verified Customer | Posted 07/05/2017
  • Human Nanog Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: stem cells
    Species: Human
    Verified Customer | Posted 12/15/2016
    Works nicely in immunostainings 1:50.
  • Human Nanog Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: stem cells
    Species: Human
    Verified Customer | Posted 12/15/2016
    Works nicely in Western blotting 1:5000.
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24178749
    Species: Human
    Verified Customer | Posted 01/06/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23910086
    Species: Human
    Verified Customer | Posted 01/06/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22851508
    Species: Other
    Verified Customer | Posted 01/06/2015
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 23778137
    Species: Human
    Verified Customer | Posted 01/06/2015

There are no reviews that match your criteria.

Showing  1 - 5 of 11 reviews Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents