Human Nanog Antibody Best Seller

Name: Human Nanog Antibody
Brand: R&D Systems
SKU: AF1997
Rating: 4 (11 reviews)

Catalog # AF1997 | R&D Systems, Inc. a Bio-Techne Brand

(11)

Citations (207)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human Nanog Antibody

Immunogen

E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376

Specificity

Detects human Nanog in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Nanog Antibody

Detection of Human Nanog by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells and Tera-2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.

Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation.

BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR.

Nanog in BG01V Human Stem Cells.

Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Nanog in BG01V Human Stem Cells-derived Embryoid Body.

Nanog was detected in immersion fixed BG01V human embryonic stem cells-derived embryoid body using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Nanog in ADLF1 and FAB2 Stem Cell Lines.

Nanog was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Nanog in Human Testicular Cancer.

Nanog was detected in immersion fixed paraffin-embedded sections of human testicular cancer using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Nanog in Human Testis.

Nanog was detected in immersion fixed paraffin-embedded sections of normal human testis using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in developing sperm cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Nanog in 13 d.p.c. mouse embryos.

Nanog was detected in immersion fixed frozen sections of 13 d.p.c. mouse embryos using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in developing CNS. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Nanog by Simple Western^TM.

Simple Western lane view shows lysates of iPSC, loaded at 0.2 mg/mL. A specific band was detected for Nanog at approximately 55 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Applications for Human Nanog Antibody

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 10⁶ cells
Sample: BG01V human embryonic stem cell chromatin, for detection see our available Human Nanog Primer Pair (Catalog # RDP-320-025)

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and immersion fixed ADLF1 and FAB2 induced pluripotent stem cell lines

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testicular cancer, immersion fixed paraffin-embedded sections of normal human testis, and immersion fixed frozen sections of 13 d.p.c. mouse embryos

Simple Western

Western Blot

1 µg/mL
Sample: BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 11 reviews rated 4 using AF1997 in the following applications:

Immunocytochemistry (1 Review)
Immunocytochemistry/Immunofluorescence (3 Reviews)
Immunofluorescence (2 Reviews)
Western Blot (5 Reviews)

Published Applications

Read 207 publications using AF1997 in the following applications:

ChIP (3 Publications)
ChIP-Seq (2 Publications)
Differentiation (1 Publication)
Flow Cytometry (27 Publications)
ICC (156 Publications)
ICC/IF (1 Publication)
IF (1 Publication)
IHC (19 Publications)
IHC-Fr (7 Publications)
IHC-P (1 Publication)
Immunoprecipitation (3 Publications)
Western Blot (18 Publications)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).

References

Mitsui, K. et al. (2003) Cell 11:631.
Chambers, I. et al. (2003) Cell 113:643.
Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Long Name

Nanog Homeobox

Alternate Names

Nanog, FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Entrez Gene IDs

79923 (Human); 71950 (Mouse)

Gene Symbol

NANOG

UniProt

ABZ92376

Product Documents for Human Nanog Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Nanog Antibody

For research use only

Citations
Reviews

Citations for Human Nanog Antibody (207)

Showing 1 - 10 of 207 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Nanog Antibody include:

Species: Human
Sample Types: Transfected Whole Cells
Applications: ICC

Valtonen et al. (2023-05-27), The Junctophilin-2 Mutation p.(Thr161Lys) Is Associated with Hypertrophic Cardiomyopathy Using Patient-Specific iPS Cardiomyocytes and Demonstrates Prolonged Action Potential and Increased Arrhythmogenicity Biomedicines
PMID: 37371654
Species: Human
Sample Types: Transfected Whole Cells
Applications: ICC

NM Schottmann et al. (2023-04-12), Generation of induced pluripotent stem cell line (UKWNLi008) derived from a patient carrying a c.1678C>G variant in the transient receptor potential cation channel subfamily A member (TRPA1) gene potentially associated with small fiber neuropathy Stem Cell Research, 2023-069(0):103094.
PMID: 37079968
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Teague et al. (2023-04-10), The time integral of BMP signaling determines fate in a stem cell model for early human development bioRxiv : the preprint server for biology, 2023-00(0).
PMID: 37090515
Species: Human

SD Sharma et al. (2023-04-04), Astrocytes mediate cell non-autonomous correction of aberrant firing in human FXS neurons Cell Reports, 2023-042(4):112344.
PMID: 37018073
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

N Son et al. (2023-03-14), Generation of a human fibroblast-derived induced pluripotent stem cell line KRIBBi009-A from a patient with breast cancer Stem Cell Research, 2023-068(0):103060.
PMID: 36925228
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Zuo Q et al. (2023-02-28), Plexin-B3 expression stimulates MET signaling, breast cancer stem cell specification, and lung metastasis. Cell Rep, 2023-042(3):112164.
PMID: 36857181
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

K Klug et al. (2023-01-09), Generation of two induced pluripotent stem cell lines UKWNLi006 and UKWNLi007 derived from two patients with an active site GLA mutation leading to a pain and no pain phenotype in Fabry disease Stem Cell Research, 2023-067(0):103025.
PMID: 36640471
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC

EY Akda? et al. (2022-12-30), CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndrome Stem Cell Research, 2022-167(0):103012.
PMID: 36610307
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Li et al. (2022-12-09), Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson's disease carrying a novel heterozygotic PARK8 (LRRK2) mutation Stem Cell Research, 2022-166(0):102995.
PMID: 36528012
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Yang et al. (2022-12-05), Generation of an induced pluripotent stem cell line (IPCASi001-A) from an autism spectrum disorder individual without intellectual disability Stem Cell Research, 2022-166(0):102994.
PMID: 36502620
Species: Human
Sample Types: Transduced Whole Cells
Applications: ICC

D Choudhury et al. (2022-11-29), Inhibition of glutaminolysis restores mitochondrial function in senescent stem cells Cell Reports, 2022-141(9):111744.
PMID: 36450260
Species: Human
Sample Types: Transfected Whole Cells
Applications: ICC

M Tongbaen et al. (2022-10-28), Generation of human induced pluripotent stem cells (CHULAi001-A) from peripheral blood mononuclear cells of a glucose-6-phosphate dehydrogenase (G6PD) deficient subject carrying the Viangchan mutation Stem Cell Research, 2022-165(0):102953.
PMID: 36334578
Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Han et al. (2022-10-06), A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants Cell Stem Cell, 2022-129(10):1475-1490.e6.
PMID: 36206731
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

R Chimienti et al. (2022-09-27), Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-040(13):111423.
PMID: 36170817
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

R Rust et al. (2022-09-16), Xeno-free induced pluripotent stem cell-derived neural progenitor cells for in vivo applications Journal of Translational Medicine, 2022-020(1):421.
PMID: 36114512
Species: Human
Sample Types: Whole Cells
Applications: IHC

T Steiner et al. (2022-09-15), RNA-based generation of iPSCs from a boy carrying the mutation m.9185ï¿½T>C in the mitochondrial gene MT-ATP6 and from his healthy mother Stem Cell Research, 2022-064(0):102920.
PMID: 36137325
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ChIP-Seq,Immunoprecipitation,ICC

Z Ai et al. (2022-08-23), Krï¿½ppel-like factor 5 rewires NANOG regulatory network to activate human naive pluripotency specific LTR7Ys and promote naive pluripotency Cell Reports, 2022-040(8):111240.
PMID: 36001968
Species: Human
Sample Types: Whole Cells
Applications: IHC

S Schmidt et al. (2022-08-16), Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease Nature Communications, 2022-013(1):4819.
PMID: 35974013
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

L László et al. (2022-08-08), A Novel Cell-Based Model for a Rare Disease: The Tks4-KO Human Embryonic Stem Cell Line as a Frank-Ter Haar Syndrome Model System International Journal of Molecular Sciences, 2022-023(15).
PMID: 35955935
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC

Z Jiang et al. (2022-08-04), Production of a human iPSC line from an early-onset Parkinson's disease patient with a novel CHCHD2 gene truncated mutation Stem Cell Research, 2022-064(0):102881.
PMID: 35944313
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

Y Sun et al. (2022-07-11), Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology Oncogene, 2022-063(0):102859.
PMID: 35870248
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

J Lotila et al. (2022-07-11), Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient Oncogene, 2022-063(0):102865.
PMID: 35843021
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

M Ohgushi et al. (2022-06-21), Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model Cell Reports, 2022-039(12):110973.
PMID: 35732132
Species: Human
Sample Types: Whole Cells
Applications: ICC

YH Chen et al. (2022-06-14), Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1 Oncogene, 2022-063(0):102845.
PMID: 35728441
Species: Human
Sample Types: Whole Tissue
Applications: IHC

M Simunovic et al. (2022-06-02), Inï¿½vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells Cell Stem Cell, 2022-029(6):962-972.e4.
PMID: 35659878
Species: Human
Sample Types: Whole Cells
Applications: ICC

H Jiao et al. (2022-05-31), Epigenetic regulation of BAF60A determines efficiency of miniature swine iPSC generation Scientific Reports, 2022-012(1):9039.
PMID: 35641537
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Fu et al. (2022-05-05), Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9 Stem Cell Research, 2022-062(0):102800.
PMID: 35561459
Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Shi et al. (2022-05-04), Generation of a human iPSC line CIBi011-A from amniocytes of a healthy fetus Stem Cell Research, 2022-062(0):102801.
PMID: 35609349
Species: Human
Sample Types: Whole Cells
Applications: ICC

X Hu et al. (2022-04-04), Generation of a human induced pluripotent stem cell line FMUPDCi001-A from a patient with mental retardation, autosomal recessive 36 (MRT36) carrying the variants c.219dupA and c.587Cï¿½>ï¿½T in ADAT3 Stem Cell Research, 2022-061(0):102777.
PMID: 35405382
Species: Human
Sample Types: Whole Cells
Applications: ICC

AJ Collier et al. (2022-03-25), Genome-wide screening identifies Polycomb repressive complex 1.3 as an essential regulator of human naï¿½ve pluripotent cell reprogramming Science Advances, 2022-08(12):eabk0013.
PMID: 35333572
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Breyer et al. (2022-03-14), Generation of the induced pluripotent stem cell line UKWNLi005-A derived from a patient with the GLA mutation c.376Aï¿½>ï¿½G of unknown pathogenicity in Fabry disease Stem Cell Research, 2022-061(0):102747.
PMID: 35325818
Species: Human
Sample Types: Whole Cells
Applications: ICC

C Lorenz et al. (2022-03-08), Generation of four iPSC lines from four patients with Leigh syndrome carrying homoplasmic mutations m.8993Tï¿½>ï¿½G or m.8993Tï¿½>ï¿½C in the mitochondrial gene MT-ATP6 Stem Cell Research, 2022-061(0):102742.
PMID: 35279592
Species: Human
Sample Types: Whole Cells
Applications: ICC

I Fagerlund et al. (2021-12-30), Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids Cells, 2021-111(1).
PMID: 35011686
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ICC,Western Blot

BE Jewell et al. (2021-12-29), Patient-derived iPSCs link elevated mitochondrial respiratory complex I function to osteosarcoma in Rothmund-Thomson syndrome PloS Genetics, 2021-117(12):e1009971.
PMID: 34965247
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Ozgencil et al. (2021-12-02), Assessing BRCA1 activity in DNA damage repair using human induced pluripotent stem cells as an approach to assist classification of BRCA1 variants of uncertain significance PLoS ONE, 2021-116(12):e0260852.
PMID: 34855882
Species: Human
Sample Types: Whole Cells
Applications: ChIP-Seq,ICC

L Pagliaroli et al. (2021-11-09), Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest formation in ARID1B-related neurodevelopmental disorders Nature Communications, 2021-112(1):6469.
PMID: 34753942
Species: Human
Sample Types: Whole Cells
Applications: IF

JR Valcourt et al. (2021-11-09), Modulating mesendoderm competence during human germ layer differentiation Cell Reports, 2021-137(6):109990.
PMID: 34758327
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

Y Shin et al. (2021-10-22), Generation of human induced pluripotent stem cell line, KRIBBi003-A, from urinary cells of a patient with glycogen storage disease type IXa Stem Cell Research, 2021-157(0):102584.
PMID: 34740118
Species: Human
Sample Types: Whole Cells
Applications: ICC

HC Ting et al. (2021-10-16), Coactivation of GSK3beta and IGF-1 Attenuates Amyotrophic Lateral Sclerosis Nerve Fiber Cytopathies in SOD1 Mutant Patient-Derived Motor Neurons Cells, 2021-110(10).
PMID: 34685754
Species: Human
Sample Types: Whole Cells
Applications: ICC

X Huang et al. (2021-08-26), OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naï¿½ve and primed pluripotent states in human Nature Communications, 2021-012(1):5123.
PMID: 34446700
Species: Human
Sample Types: Whole Cells
Applications: ICC/IF

L Krumm et al. (2021-08-26), Generation and characterization of an endogenously tagged SPG11-human iPSC line by CRISPR/Cas9 mediated knock-in Stem Cell Research, 2021-056(0):102520.
PMID: 34479069
Species: Human
Sample Types: Organoid
Applications: ICC

Y Kang et al. (2021-08-19), A human forebrain organoid model of fragile X syndrome exhibits altered neurogenesis and highlights new treatment strategies Nature Neuroscience, 2021-00(0).
PMID: 34413513
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC

NJ Hogrebe et al. (2021-08-04), Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines Nature Protocols, 2021-00(0).
PMID: 34349281
Species: Human
Sample Types: Whole Cells
Applications: ICC

CEM Aarts et al. (2021-06-29), Generation and characterization of a human iPSC line SANi006-A from a Gray Platelet Syndrome patient Stem Cell Research, 2021-055(0):102443.
PMID: 34237592
Species: Human
Sample Types: Whole Cells
Applications: ICC

EC Pandolfi et al. (2021-06-24), Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research Stem Cell Research, 2021-054(0):102446.
PMID: 34216981
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Ma et al. (2021-06-18), Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls Stem Cell Research, 2021-054(0):102435.
PMID: 34182254
Species: Human
Sample Types: Whole Cells
Applications: IHC

EC Pandolfi et al. (2021-06-08), Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research Stem Cell Research, 2021-054(0):102409.
PMID: 34130154
Species: Human
Sample Types: Whole Cells
Applications: ICC

PS Huang et al. (2021-06-06), Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1 Stem Cell Research, 2021-054(0):102415.
PMID: 34118566
Species: Human
Sample Types: Whole Cells
Applications: ICC

DJ Truong et al. (2021-06-03), Non-invasive and high-throughput interrogation of exon-specific isoform expression Nature Cell Biology, 2021-023(6):652-663.
PMID: 34083785
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Borsoi et al. (2021-05-25), Generation of two human induced pluripotent stem cell (hiPSC) lines derived from unrelated Marfan Syndrome patients Stem Cell Research, 2021-054(0):102407.
PMID: 34062330
Species: Human
Sample Types: Whole Cells
Applications: ICC

BSJ Low et al. (2021-05-25), Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant &beta cells Nature Communications, 2021-012(1):3133.
PMID: 34035238
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

R Yan et al. (2021-05-24), Generation of a human induced pluripotent stem cell line (SMUSHi001-A) from a patient with 46, XX male sex reversal syndrome carrying the SRY gene Stem Cell Research, 2021-054(0):102397.
PMID: 34098200
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MS Lee et al. (2021-05-19), Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration Science Advances, 2021-07(21).
PMID: 34138734
Species: Human
Sample Types: Embryo
Applications: IHC

D Meisterman et al. (2021-05-17), Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification Cell Stem Cell, 2021-00(0).
PMID: 34004179
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

S Wu et al. (2021-05-16), Generation of a human iPSC line QDMHi001-A from a patient with Marfan syndrome carrying a heterozygous c.6772ï¿½Tï¿½>ï¿½C variant in FBN1 Stem Cell Research, 2021-054(0):102390.
PMID: 34087733
Species: Human
Sample Types: Cell Lysates
Applications: ChIP

J Bayerl et al. (2021-04-28), Principles of signaling pathway modulation for enhancing human naive pluripotency induction Cell Stem Cell, 2021-00(0).
PMID: 33915080
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Mertens et al. (2021-04-27), Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patients Cell Stem Cell, 2021-00(0).
PMID: 33910058
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC

W Ge et al. (2021-04-20), Generation of a human iPSC line CIBi009-A from a patient with familial hypercholesterolemia carrying variants of LDLR c.T1241G and APOB c.G1618T Stem Cell Research, 2021-053(0):102347.
PMID: 33892292
Species: Human
Sample Types: Whole Cells
Applications: ICC

DJ Sutcliffe et al. (2021-04-19), Induced pluripotent stem cells from subjects with Lesch-Nyhan disease Scientific Reports, 2021-011(1):8523.
PMID: 33875724
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ICC,Flow Cytometry,Western Blot

N Raj et al. (2021-04-13), Cell-type-specific profiling of human cellular models of fragile X syndrome reveal PI3K-dependent defects in translation and neurogenesis Cell Reports, 2021-035(2):108991.
PMID: 33852833
Species: Human
Sample Types: Whole Cells
Applications: ICC

G Guo et al. (2021-04-07), Human naive epiblast cells possess unrestricted lineage potential Cell Stem Cell, 2021-00(0).
PMID: 33831366
Species: Human
Sample Types: Whole Cells
Applications: ICC

G Inak et al. (2021-03-26), Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome Nature Communications, 2021-012(1):1929.
PMID: 33771987
Species: Human
Sample Types: Whole Cells
Applications: ICC

R Hanna et al. (2021-03-23), G-quadruplexes originating from evolutionary conserved L1 elements interfere with neuronal gene expression in Alzheimer's disease Nature Communications, 2021-012(1):1828.
PMID: 33758195
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Kim et al. (2021-03-22), Dysregulation of mitochondria-lysosome contacts by GBA1 dysfunction in dopaminergic neuronal models of Parkinson's disease Nature Communications, 2021-012(1):1807.
PMID: 33753743
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC

LG Devito et al. (2021-03-20), Generation of an iPSC line (CRICKi001-A) from an individual with a germline SMARCA4 missense mutation and autism spectrum disorder Stem Cell Research, 2021-053(0):102304.
PMID: 33799280
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Bellák et al. (2020-12-29), Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment Scientific Reports, 2020-110(1):22414.
PMID: 33376249
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

E Hathy et al. (2020-12-25), Generation of multiple iPSC clones from a male schizophrenia patient carrying de novo mutations in genes KHSRP, LRRC7, and KIR2DL1, and his parents Stem Cell Research, 2020-151(0):102140.
PMID: 33503521
Species: Human
Sample Types: Whole Cells
Applications: ICC

DX Medina et al. (2020-12-24), Generation of two induced pluripotent stem cell (iPSC) lines from an ALS patient with simultaneous mutations in KIF5A and MATR3 genes Stem Cell Research, 2020-150(0):102141.
PMID: 33388707
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

M Zhou et al. (2020-12-10), Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs Stem Cell Research, 2020-150(0):102120.
PMID: 33352530
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Carlson-St et al. (2020-12-08), Design of efficacious somatic cell genome editing strategies for recessive and polygenic diseases Nature Communications, 2020-111(1):6277.
PMID: 33293555
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

V Cerrada et al. (2020-12-03), Generation of the iPSC line IISHDOi007-A from peripheral blood mononuclear cells from a patient with McArdle disease harbouring the mutation c.2392ï¿½Tï¿½>ï¿½C; p.Trp798Arg Stem Cell Research, 2020-149(0):102108.
PMID: 33370875
Species: Human
Sample Types: Whole Cells
Applications: ICC

B Kuebler et al. (2020-11-19), Derivation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering 7q11.23 microduplication syndrome Stem Cell Res, 2020-149(0):102092.
PMID: 33254092
Species: Human
Sample Types: Whole Cells
Applications: ICC

R Yan et al. (2020-11-19), Generation of a human induced pluripotent stem cell line (SBWCHi001-A) from a patient with NEDSDV carrying a pathogenic mutation in CTNNB1 gene Stem Cell Res, 2020-149(0):102091.
PMID: 33264726
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

B Kuebler et al. (2020-11-16), Generation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering Williams-Beuren syndrome (7q11.23 deletion) Stem Cell Research, 2020-149(0):102087.
PMID: 33370870
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Inglés-Fer et al. (2020-11-07), Generation, establishment and characterization of a pluripotent stem cell line (CVTTHi001-A) from primary fibroblasts isolated from a patient with activated PI3 kinase delta syndrome (APDS2) Stem Cell Res, 2020-149(0):102082.
PMID: 33221676
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Wu et al. (2020-10-29), Generation of a human iPSC line CIBi008-A from amniotic fluid-derived cells of a fetus with &beta-thalassemia carrying variants of -28Aï¿½>ï¿½G and IVS-II-654Cï¿½>ï¿½T in HBB Stem Cell Res, 2020-149(0):102074.
PMID: 33157391
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

DH Kim et al. (2020-10-02), Generation of a human induced pluripotent stem cell line, YCMi002-A, from a Factor VII deficiency patient carrying F7 mutations Stem Cell Res, 2020-149(0):102026.
PMID: 33038747
Species: Human
Sample Types: Whole Cells
Applications: ICC

SR Sripathy et al. (2020-09-17), Generation of 10 patient-specific induced pluripotent stem cells (iPSCs) to model Pitt-Hopkins Syndrome Stem Cell Res, 2020-048(0):102001.
PMID: 32971458
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Shi et al. (2020-09-15), Generation of hypoimmunogenic human pluripotent stem cells via expression of membrane-bound and secreted &beta2m-HLA-G fusion proteins Stem Cells, 2020-00(0).
PMID: 32930470
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Rolova et al. (2020-09-02), Generation of a human induced pluripotent stem cell line (UEFi003-A) carrying heterozygous A673T variant in amyloid precursor protein associated with a reduced risk of Alzheimer's disease Stem Cell Res, 2020-048(0):101968.
PMID: 32911327
Species: Human
Sample Types: Whole Cells,Whole Tissue
Applications: ICC,IHC

MN Shahbazi et al. (2020-08-10), Developmental potential of aneuploid human embryos cultured beyond implantation Nat Commun, 2020-011(1):3987.
PMID: 32778678
Species: Mouse
Sample Types: Whole Tissue,Whole Cells
Applications: ICC,IHC

L Muzio et al. (2020-07-31), Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis Nat Commun, 2020-011(1):3848.
PMID: 32737286
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

T Boerstler et al. (2020-06-29), CRISPR/Cas9 mediated generation of human ARID1B heterozygous knockout hESC lines to model Coffin-Siris syndrome Stem Cell Res, 2020-047(0):101889.
PMID: 32682288
Species: Human
Sample Types: Whole Cells
Applications: ICC

I Buers et al. (2020-06-01), Crisponi syndrome/cold-induced sweating syndrome type 2: Reprogramming of CS/CISS2 individual derived fibroblasts into three clones of one iPSC line Stem Cell Res, 2020-046(0):101855.
PMID: 32512309
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC

L Schöning et al. (2020-05-28), Generation of induced pluripotent stem cell lines from a Crisponi/Cold induced sweating syndrome type 1 individual Stem Cell Res, 2020-046(0):101820.
PMID: 32492556
Species: Human
Sample Types: Whole Cells
Applications: ICC

V Alexeeva et al. (2020-05-06), A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing Stem Cell Res, 2020-045(0):101837.
PMID: 32413789
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

W Xie et al. (2020-04-27), The H2B ubiquitin-protein ligase RNF40 is required for somatic cell reprogramming Cell Death Dis, 2020-011(4):287.
PMID: 32341358
Species: Human
Sample Types: Whole Cells
Applications: ICC

K Zhang et al. (2020-04-21), Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation Cell Rep, 2020-031(3):107521.
PMID: 32320667
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Frew et al. (2020-03-16), Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency Mol Neurodegener, 2020-015(1):21.
PMID: 32178712
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Zhang et al. (2020-02-20), Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi004-A) from a Wilson's disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene Stem Cell Res, 2020-044(0):101741.
PMID: 32113134
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Frew et al. (2019-11-08), Generation of an induced pluripotent stem cell line (UBCi001-A) from a presymptomatic individual carrying the R418X progranulin gene mutation Stem Cell Res, 2019-141(0):101582.
PMID: 31707213
Species: Human
Sample Types: Whole Cells
Applications: ICC

N Bredenkamp et al. (2019-11-07), Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency Stem Cell Reports, 2019-113(6):1083-1098.
PMID: 31708477
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Stubb et al. (2019-10-18), Superresolution architecture of cornerstone focal adhesions in human pluripotent stem cells Nat Commun, 2019-110(1):4756.
PMID: 31628312
Species: Human
Sample Types: Whole Cells
Applications: ICC

MH Wen et al. (2019-10-16), Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1 Stem Cell Res, 2019-141(0):101631.
PMID: 31704540
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Jang et al. (2019-09-26), Control over single-cell distribution of G1 lengths by WNT governs pluripotency PLoS Biol., 2019-017(9):e3000453.
PMID: 31557150
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

EC Pandolfi et al. (2019-09-16), Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research Stem Cell Res, 2019-040(0):101576.
PMID: 31622877
Species: Human
Sample Types: Whole Cells
Applications: IHC

T Hiramoto et al. (2019-09-12), Non-transmissible MV Vector with Segmented RNA Genome Establishes Different Types of iPSCs from Hematopoietic Cells Mol. Ther., 2019-028(1):129-141.
PMID: 31677955
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Tiihonen et al. (2019-09-02), Sex-specific transcriptional and proteomic signatures in schizophrenia Nat Commun, 2019-010(1):3933.
PMID: 31477693
Species: Human
Sample Types: Whole Cells
Applications: ICC

V Cerrada et al. (2019-08-29), Derivation of a human DOA iPSC line, IISHDOi006-A, with a mutation in the ACO2 gene: c.1999G>A; p.Glu667Lys Stem Cell Res, 2019-040(0):101566.
PMID: 31509793
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Shan et al. (2019-08-26), Tcf7l1 acts as a suppressor for the self-renewal of liver cancer stem cells and is regulated by IGF/MEK/ERK signaling independent ofÎ²-catenin Stem Cells, 2019-00(0).
PMID: 31322782
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Park et al. (2019-07-18), Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23) Stem Cell Res, 2019-039(0):101499.
PMID: 31352199
Species: Human
Sample Types: Whole Tissue
Applications: IHC

M Huang et al. (2019-06-13), Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis Cell Stem Cell, 2019-00(0).
PMID: 31204176
Species: Human
Sample Types: Whole Cells
Applications: ICC

N Kolundzic et al. (2019-05-13), Induced pluripotent stem cell line heterozygous for p.R2447X mutation in filaggrin: KCLi002-A Stem Cell Res, 2019-038(0):101462.
PMID: 31103941
Species: Human
Sample Types: Whole Cells
Applications: ICC

C Calatayud et al. (2019-05-02), CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons Sci Rep, 2019-09(1):6811.
PMID: 31048719
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Neri et al. (2019-04-26), Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis Nat Commun, 2019-010(1):1929.
PMID: 31028265
Species: Human
Sample Types: Whole Cells
Applications: ICC

N Kolundzic et al. (2019-03-27), Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype Stem Cell Res, 2019-037(0):101424.
PMID: 30933721
Species: Human
Sample Types: Whole Tissue,Exosomes
Applications: IHC,Western Blot

M Sherman-Sa et al. (2019-03-21), SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma Gynecol. Oncol., 2019-00(0).
PMID: 30904337
Species: Human
Sample Types: Whole Cells
Applications: ICC

F Tsurumi et al. (2019-03-15), The intracellular Ca2+ concentration is elevated in cardiomyocytes differentiated from hiPSCs derived from a Duchenne muscular dystrophy patient PLoS ONE, 2019-014(3):e0213768.
PMID: 30875388
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Elkouris et al. (2019-02-22), Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson's Disease Patients Front Cell Neurosci, 2019-013(0):58.
PMID: 30853899
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Wang et al. (2019-02-07), Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development JCI Insight, 2019-04(3).
PMID: 30728336
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

X Wang et al. (2019-01-09), Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale PeerJ, 2019-06(0):e6143.
PMID: 30643676
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

S Frank et al. (2018-12-13), yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs Cell Stem Cell, 2018-10(0).
PMID: 30554961
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Cells
Applications: ICC

Y Kang et al. (2018-11-27), Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys Cell Rep, 2018-125(9):2563-2576.e9.
PMID: 30485820
Species: Human
Sample Types: Whole Cells
Applications: ICC

H Jeon et al. (2018-11-20), Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing J Clin Med, 2018-17(11).
PMID: 30463279
Species: Human
Sample Types: Whole Cells
Applications: Differentiation

S Kaur et al. (2018-10-19), Small non-coding RNA landscape of extracellular vesicles from human stem cells Sci Rep, 2018-18(1):15503.
PMID: 30341351
Species: Human
Sample Types: Whole Cells
Applications: ICC

A Yoney et al. (2018-10-12), WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids Elife, 2018-17(0).
PMID: 30311909
Species: Human
Sample Types: Whole Cells
Applications: ICC

F Erdlenbruc et al. (2018-10-06), Generation of induced pluripotent stem cells (iPSCs) from human foreskin fibroblasts Stem Cell Res, 2018-133(0):79-82.
PMID: 30321832
Species: Human
Sample Types: Whole Cells
Applications: IHC

Y Zhang et al. (2018-09-20), 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling Cell Stem Cell, 2018-00(0).
PMID: 30244870
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Devito et al. (2018-07-25), Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A Stem Cell Res, 2018-031(0):122-126.
PMID: 30075366
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Immunoprecipitation,Western Blot,ICC

TS Barakat et al. (2018-07-19), Functional Dissection of the Enhancer Repertoire in Human Embryonic Stem Cells Cell Stem Cell, 2018-023(2):276-288.e8.
PMID: 30033119
Species: Human
Sample Types: Whole Cells
Applications: ICC

SY Lee et al. (2018-06-09), Generation of gene-corrected iPSC line from Parkinson's disease patient iPSC line with alpha-SNCA A53T mutation Stem Cell Res, 2018-030(0):145-149.
PMID: 29906669
Species: Human
Sample Types: Whole Cells
Applications: ICC

G Stratigopo et al. (2018-06-04), DMSO increases efficiency of genome editing at two non-coding loci PLoS ONE, 2018-013(6):e0198637.
PMID: 29864154
Species: Human
Sample Types: Whole Cells
Applications: ICC

Š Lehtonen et al. (2018-05-19), Generation of a human induced pluripotent stem cell line from a patient with a rare A673T variant in amyloid precursor protein gene that reduces the risk for Alzheimer's disease Stem Cell Res, 2018-030(0):96-99.
PMID: 29807259
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

RK Jangid et al. (2018-05-02), Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions BMC Genomics, 2018-019(1):313.
PMID: 29716520
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

WA Pastor et al. (2018-04-25), TFAP2C regulates transcription in human naive pluripotency by opening enhancers Nat. Cell Biol., 2018-020(5):553-564.
PMID: 29695788
Species: Human
Sample Types: Whole Cells
Applications: ICC

PJ Yang et al. (2018-03-22), Biological characterization of human amniotic epithelial cells in a serum-free system and their safety evaluation Acta Pharmacol. Sin., 2018-00(0).
PMID: 29565036
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Shafa et al. (2018-03-15), Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers Front Med (Lausanne), 2018-05(0):69.
PMID: 29600249
Species: Human
Sample Types: Whole Cells
Applications: ICC

I Kogut et al. (2018-02-21), High-efficiency RNA-based reprogramming of human primary fibroblasts Nat Commun, 2018-09(1):745.
PMID: 29467427
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

OM Russell et al. (2018-01-29), Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo Sci Rep, 2018-08(1):1799.
PMID: 29379065
Species: Human
Sample Types: Whole Cells
Applications: ICC

CW Chang et al. (2017-12-23), Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation Stem Cell Res, 2017-127(0):21-24.
PMID: 29291512
Species: Human
Sample Types: Whole Cells
Applications: ICC

G Xu et al. (2017-12-13), Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing Stem Cell Res, 2017-127(0):38-41.
PMID: 29310060
Species: Human
Sample Types: Whole Cells
Applications: ICC

N Yahata et al. (2017-11-14), TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation Sci Rep, 2017-17(1):15557.
PMID: 29138463
Species: Human
Sample Types: Whole Cells
Applications: ICC

B Kuebler et al. (2017-09-09), Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS) Stem Cell Res, 2017-00(0).
PMID: 29150092
Species: Human
Sample Types: Whole Cells
Applications: ICC

CA Thomas et al. (2017-08-10), Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation Cell Stem Cell, 2017-00(0).
PMID: 28803918
Species: Human
Sample Types: Whole Cells
Applications: ICC

E Baljinnyam et al. (2017-08-01), Effect of densely ionizing radiation on cardiomyocyte differentiation from human-induced pluripotent stem cells Physiol Rep, 2017-05(15).
PMID: 28801517
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Klein et al. (2017-07-03), A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling Stem Cell Res, 2017-023(0):73-76.
PMID: 28925368
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Miquel-Ser et al. (2017-06-07), Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines Stem Cell Res, 2017-024(0):155-159.
PMID: 29034884
Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Xu et al. (2017-05-19), Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9 Stem Cell Res, 2017-022(0):29-32.
PMID: 28952928
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

MJ Bouma et al. (2017-05-09), Differentiation-Defective Human Induced Pluripotent Stem Cells Revealï¿½Strengths and Limitations of the Teratoma Assay and Inï¿½Vitro Pluripotency Assays Stem Cell Reports, 2017-08(5):1340-1353.
PMID: 28494940
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: ICC,Western Blot

P Wang et al. (2017-04-25), HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 2017-08(17):28074-28092.
PMID: 28427209
Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Miyagoe-Su et al. (2017-04-12), Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status Stem Cells Int, 2017-02017(0):7906843.
PMID: 28491099
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow Cytometry

F Sampazioti et al. (2017-03-23), Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells Nat Protoc, 2017-012(4):814-827.
PMID: 28333915
Species: Human
Sample Types: Whole Cells
Applications: ICC

E Varga et al. (2017-03-18), Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) Stem Cell Res, 2017-021(0):19-22.
PMID: 28677532
Species: Human
Sample Types: Transduced Whole Cells
Applications: ICC

Z Geng et al. (2017-03-10), Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration PLoS ONE, 2017-012(3):e0173575.
PMID: 28282420
Species: Human
Sample Types: Whole Cells
Applications: ICC

I Karakikes et al. (2017-02-28), A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases Circ. Res, 2017-00(0).
PMID: 28246128
Species: Human
Sample Types: Whole Cells
Applications: ICC

C Lorenz et al. (2017-01-26), Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders Cell Stem Cell, 2017-00(0).
PMID: 28132834
Species: Human
Sample Types: Whole Cells
Applications: ICC

JS Saini et al. (2017-01-26), Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration Cell Stem Cell, 2017-00(0).
PMID: 28132833
Species: Human
Sample Types: Whole Cells
Applications: ICC

Florian Wegner (2016-11-23), Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization J. Neurosci., 2016-136(47):12027-12043.
PMID: 27881786
Species: Human
Sample Types: Whole Cells
Applications: ICC

N Zeltner et al. (2016-11-14), Capturing the biology of disease severity in a PSC-based model of familial dysautonomia Nat. Med., 2016-122(12):1421-1427.
PMID: 27841875
Species: Human
Sample Types: Whole Cells
Applications: ICC

(2016-10-08), Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton's jelly and bone marrow FEBS Open Bio, 2016-16(11):1054-1066.
PMID: 27833846
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: IHC-Fr

(2016-09-17), An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts Stem Cell Res, 2016-017(2):444-447.
PMID: 27879222
Species: Human
Sample Types: Cell Culture Supernates
Applications: IHC

(2016-09-08), A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells PLoS ONE, 2016-011(9):e0161229.
PMID: 27606941
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

(2016-09-01), Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells Stem Cell Reports, 2016-07(3):496-507.
PMID: 27594587
Species: Human
Sample Types: Whole Cells
Applications: IHC

(2016-08-26), Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application Stem Cell Res, 2016-017(2):379-390.
PMID: 27639108
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

(2016-08-22), Generation of HEXA-deficient hiPSCs from fibroblasts of a Tay-Sachs disease patient Stem Cell Res, 2016-017(2):289-291.
PMID: 27879213
Species: Human
Sample Types: Whole Cells
Applications: ICC

(2016-07-26), A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients Elife, 2016-05(0).
PMID: 27458797
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

C Eguizabal (2016-06-30), Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming Stem Cells, 2016-00(0).
PMID: 27300161
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

O Guillevic et al. (2016-04-04), A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity PLoS ONE, 2016-011(4):e0152993.
PMID: 27043207
Species: Human
Sample Types: Whole Cells
Applications: ICC

Freibaum B et al. (2015-08-26), GGGGCC repeat expansion in C9orf72 compromises nucleocytoplasmic transport. Nature, 2015-0525(7567):129-33.
PMID: 26308899
Species: Human
Sample Types: Whole Cells
Applications: IHC

Ma H et al. (2015-07-15), Metabolic rescue in pluripotent cells from patients with mtDNA disease. Nature, 2015-0524(7564):234-8.
PMID: 26176921
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Francescangeli F et al. (2015-03-20), Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer. Cell Death Differ, 2015-022(10):1700-13.
PMID: 26343543
Species: Human
Sample Types: Whole Cells
Applications: ICC

Pino-Barrio M et al. (2015-03-12), V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits. PLoS ONE, 2015-010(3):e0118499.
PMID: 25764185
Species: Human
Sample Types: Whole Cells
Applications: ICC

Liu L et al. (2015-01-08), Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability. Stem Cells Dev, 2015-024(8):962-72.
PMID: 25422984
Species: Human
Sample Types: Whole Cells
Applications: ICC

Bayin et al. (2014-12-26), Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells. PLoS ONE, 2014-19(12):e116114.
PMID: 25541984
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Jia D et al. (2014-09-26), beta-Catenin and NF-kappaB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer. Cell Death Differ, 2014-022(2):298-310.
PMID: 25257174
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Wu et al. (2014-08-14), Effects of pulsed electromagnetic field on differentiation of HUES-17 human embryonic stem cell line. Int J Mol Sci, 2014-015(8):14180-90.
PMID: 25196518
Species: Human
Sample Types: Whole Cells
Applications: ICC

Kobayashi et al. (2014-07-22), Directed differentiation of patient-specific induced pluripotent stem cells identifies the transcriptional repression and epigenetic modification of NKX2-5, HAND1, and NOTCH1 in hypoplastic left heart syndrome. PLoS ONE, 2014-09(7):e102796.
PMID: 25050861
Species: Human
Sample Types: Whole Cells
Applications: ICC

Piao Y et al. (2014-06-05), Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors. Stem Cells Transl Med, 2014-03(0):787-791.
PMID: 24904173
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

Ferrer M et al. (2014-05-29), A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium. Stem Cells Transl Med, 2014-03(8):911-22.
PMID: 24873859
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Chaurasia P et al. (2014-04-24), Epigenetic reprogramming induces the expansion of cord blood stem cells. J Clin Invest, 2014-0124(6):2378-95.
PMID: 24762436
Species: Human
Sample Types: Whole Cells
Applications: ICC

Wada et al. (2014-02-10), Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. Stem Cells Dev, 2014-023(18):2225-36.
PMID: 24380401
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ICC,Western Blot

Luo et al. (2014-01-21), Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells. Sci Rep, 2014-04(0):3779.
PMID: 24445363
Species: Human
Sample Types: Whole Cells
Applications: ICC

Bershteyn et al. (2014-01-12), Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells. Nature, 2014-0507(7490):99-103.
PMID: 24413397
Species: Human
Sample Types: Whole Cells
Applications: ICC

Sproul A et al. (2014-01-08), Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors. PLoS ONE, 2014-09(1):e84547.
PMID: 24416243
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Piva M et al. (2014-01-01), Sox2 promotes tamoxifen resistance in breast cancer cells. EMBO Mol Med, 2014-06(1):66-79.
PMID: 24178749
Species: Human
Sample Types: Whole Cells
Applications: ICC

Fatima A et al. (2013-12-11), The disease-specific phenotype in cardiomyocytes derived from induced pluripotent stem cells of two long QT syndrome type 3 patients. PLoS ONE, 2013-18(12):e83005.
PMID: 24349418
Species: Human
Sample Types: Whole Cells
Applications: ICC

Bellin et al. (2013-11-08), Isogenic human pluripotent stem cell pairs reveal the role of a KCNH2 mutation in long-QT syndrome. EMBO J, 2013-132(24):3161-75.
PMID: 24213244
Species: Primate - Pan troglodytes (Chimpanzee),Human,Primate - Pan paniscus (Bonobo)
Sample Types: Whole Cells
Applications: ICC

Marchetto M et al. (2013-10-23), Differential L1 regulation in pluripotent stem cells of humans and apes. Nature, 2013-1503(7477):525-9.
PMID: 24153179
Species: Human
Sample Types: Whole Cells
Applications: ICC

Nathwani B et al. (2013-10-08), Morphological differences of primary cilia between human induced pluripotent stem cells and their parental somatic cells. Stem Cells Dev, 2013-123(2):115-23.
PMID: 24007236
Species: Human
Sample Types: Whole Cells
Applications: ICC

Duggal G et al. (2013-08-14), Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential. Stem Cells Dev, 2013-022(23):3141-55.
PMID: 23829223
Species: Human
Sample Types: Whole Cells
Applications: IHC

Yoshioka N et al. (2013-08-01), Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell, 2013-013(2):246-54.
PMID: 23910086
Species: Human
Sample Types: Whole Cells
Applications: ICC

Zhang R et al. (2013-01-01), A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells. Nat Commun, 2013-04(0):1335.
PMID: 23299885
Species: Human
Sample Types: Whole Cells
Applications: ICC

Hayashi et al. (2012-09-24), Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. PLoS ONE, 2012-07(9):e45435.
PMID: 23029008
Species: Human
Sample Types: Whole Cells
Applications: ICC

Pekkanen-Mattila M et al. (2012-01-19), The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells. Stem Cells Int, 2012-02012(0):875059.
PMID: 22315618
Species: Human
Sample Types: Cell Lysates
Applications: ChIP

Wong RC et al. (2011-10-01), A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells. Stem Cells, 2011-129(10):1517-27.
PMID: 21898682
Species: Human
Sample Types: Whole Cells
Applications: ICC

Kim SY et al. (2011-08-29), Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells. Stem Cells Dev., 2011-021(8):1272-86.
PMID: 21787230
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Leis O et al. (2011-08-08), Sox2 expression in breast tumours and activation in breast cancer stem cells. Oncogene, 2011-031(11):1354-65.
PMID: 21822303
Species: Human
Sample Types: Whole Cells
Applications: ICC

Emdad L et al. (2011-07-26), Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes. Stem Cells Dev., 2011-021(3):404-10.
PMID: 21631388
Species: Human
Sample Types: Whole Cells
Applications: ICC

Jiang W et al. (2011-04-01), CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells. Stem Cells, 2011-029(4):609-17.
PMID: 21308865
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Plews JR et al. (2010-12-30), Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach. PLoS ONE, 2010-15(12):e14397.
PMID: 21209933
Species: Human
Sample Types: Whole Cells
Applications: ICC

Klim JR et al. (2010-11-14), A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat. Methods, 2010-17(12):989-94.
PMID: 21076418
Species: Human
Sample Types: Whole Cells
Applications: ICC

Carvajal-Vergara X et al. (2010-06-10), Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome. Nature, 2010-0465(7299):808-12.
PMID: 20535210
Species: Human
Sample Types: Cell Lysates
Applications: ChIP

Chng Z et al. (2010-01-08), SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells. Cell Stem Cell, 2010-06(1):59-70.
PMID: 20074535
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: Western Blot,ICC

Chayosumrit M et al. (2009-10-14), Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm. Biomaterials, 2009-131(3):505-14.
PMID: 19833385
Species: Human
Sample Types: Whole Cells
Applications: ICC

Ye L et al. (2009-05-29), Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases. Proc. Natl. Acad. Sci. U.S.A., 2009-0106(24):9826-30.
PMID: 19482945
Species: Human
Sample Types: Whole Cells
Applications: ICC

Chang CW et al. (2009-05-01), Polycistronic lentiviral vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem Cells, 2009-027(5):1042-9.
PMID: 19415770
Species: Human
Sample Types: Whole Cells
Applications: ICC

Thomas RJ et al. (2009-04-15), Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Biotechnol. Bioeng., 2009-0102(6):1636-44.
PMID: 19062183
Species: Human
Sample Types: Whole Cells
Applications: ICC

(2009-04-01), The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used. J. Cell. Biochem., 2009-0106(5):867-77.
PMID: 19199344
Species: Human
Sample Types: Whole Cells
Applications: ICC

Taura D et al. (2009-02-27), Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells. FEBS Lett., 2009-0583(6):1029-33.
PMID: 19250937
Species: Human
Sample Types: Whole Cells
Applications: ICC

Gil JE et al. (2009-01-20), Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett., 2009-0583(3):561-7.
PMID: 19162023
Species: Human
Sample Types: Whole Cells
Applications: ICC

Desbordes SC et al. (2008-06-05), High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Cell Stem Cell, 2008-02(6):602-12.
PMID: 18522853
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ICC,Western Blot

Takahashi K et al. (2007-11-30), Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 2007-1131(5):861-72.
PMID: 18035408
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: ICC

Navara CS et al. (2007-07-19), Pedigreed primate embryonic stem cells express homogeneous familial gene profiles. Stem Cells, 2007-025(11):2695-704.
PMID: 17641389
Species: Human
Sample Types: Whole Cells
Applications: ICC

Yu H et al. (2006-06-01), Isolation of a novel population of multipotent adult stem cells from human hair follicles. Am. J. Pathol., 2006-0168(6):1879-88.
PMID: 16723703
Species: Human
Sample Types: Whole Cells
Applications: ICC

Tsai MS et al. (2005-11-23), Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells. Biol. Reprod., 2005-174(3):545-51.
PMID: 16306422
Species: Human
Sample Types: Whole Cells
Applications: ICC

Noaksson K et al. (2005-08-04), Monitoring differentiation of human embryonic stem cells using real-time PCR. Stem Cells, 2005-023(10):1460-7.
PMID: 16081663
Species: Human
Sample Types: Whole Tissue,Cell Lysates,Whole Cells
Applications: ICC,IHC,Western Blot

Hyslop L et al. (2005-06-27), Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages. Stem Cells, 2005-023(8):1035-43.
PMID: 15983365

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Human Nanog Antibody

Name: Anonymous

Application: Immunocytochemistry/Immunofluorescence

Sample Tested: human induced pluripotent stem cells

Species: Human

Verified Customer | Posted 10/24/2023

Nanog IF staining was performed on iPSCs.
Human Nanog Antibody

Name: Anonymous

Application: Western Blot

Sample Tested: MCF7

Species: Human

Verified Customer | Posted 12/02/2018
Human Nanog Antibody

Name: Leslie Priddy

Application: Western Blot

Sample Tested: Tumor cell lyastes

Species: Human

Verified Customer | Posted 04/13/2018
Human Nanog Antibody

Name: Anonymous

Application: Immunocytochemistry/Immunofluorescence

Sample Tested: Embryonic stem cells

Species: Human

Verified Customer | Posted 11/23/2017
Human Nanog Antibody

Name: Anonymous

Application: Western Blot

Sample Tested: 7TD1 mouse hybridoma cell line

Species: Mouse

Verified Customer | Posted 07/05/2017
Human Nanog Antibody

Name: Anonymous

Application: Immunocytochemistry/Immunofluorescence

Sample Tested: stem cells

Species: Human

Verified Customer | Posted 12/15/2016

Works nicely in immunostainings 1:50.
Human Nanog Antibody

Name: Anonymous

Application: Western Blot

Sample Tested: stem cells

Species: Human

Verified Customer | Posted 12/15/2016

Works nicely in Western blotting 1:5000.
Name: Anonymous

Application: Western Blot

Sample Tested: See PMID 24178749

Species: Human

Verified Customer | Posted 01/06/2015
Name: Anonymous

Application: Immunofluorescence

Sample Tested: See PMID 23910086

Species: Human

Verified Customer | Posted 01/06/2015
Name: Anonymous

Application: Immunofluorescence

Sample Tested: See PMID 22851508

Species: Other

Verified Customer | Posted 01/06/2015
Name: Anonymous

Application: Immunocytochemistry

Sample Tested: See PMID 23778137

Species: Human

Verified Customer | Posted 01/06/2015

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Detection of Human Nanog by Western Blot.

Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation.

Nanog in BG01V Human Stem Cells.

Nanog in BG01V Human Stem Cells-derived Embryoid Body.

Nanog in ADLF1 and FAB2 Stem Cell Lines.

Nanog in Human Testicular Cancer.

Nanog in Human Testis.

Nanog in 13 d.p.c. mouse embryos.

Detection of Human Nanog by Simple WesternTM.

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