Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5469
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot, Immunocytochemistry
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 706824
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human N-Acetylglucosaminyltransferase V/MGAT5
Leu189-Leu741
Accession # Q09328
Leu189-Leu741
Accession # Q09328
Specificity
Detects human N-Acetylglucosaminyltransferase V/MGAT5 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
Detection of Human N‑Acetylglucosaminyltransferase V/MGAT5 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N-Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
N‑Acetylglucosaminyltransferase V/MGAT5 in MCF‑7 Human Cell Line.
N-Acetylglucosaminyltransferase V/MGAT5 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of N-Acetylglucosaminyltransferase V/MGAT5 by Western Blot
Knockdown of Mgat5 expression in cultured human RPE cells attenuates binding of Gal-3 to RPE cells.(A) Western blot analysis of Mgat5 expression in RPE cells transfected with siRNA containing the same nucleotides as Mgat5 siRNA in random order (sc siRNA), and the same cell line transfected with 50 pmol and 100 pmol of double stranded siRNA complementary to Mgat5 (Mgat5 siRNA), respectively. Lysates containing approximately equal amounts of protein were separated by SDS-PAGE and blotted for immunochemical detection of Mgat5 content. Experiments were repeated at least three times. MW; molecular weight. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26760037), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 1 review rated 4 using MAB5469 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: N-Acetylglucosaminyltransferase V/MGAT5
N-Acetylglucosaminyltransferase V (GnT-V), also known as mannosylglycoprotein N-acetyl-glucosaminyltransferase 5 (MGAT5), adds an N-acetylglucosamine to the alpha1-6-linked core mannose of an N-linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta1-6GlcNAc-branched arm in N-glycans. The degree of N-glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT-V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3-5).
References
- Saito, H. et al. (1994) Biochem. Biophys. Res. Commun. 198:318.
- Lau, K.S. et al. (2007) Cell 198:123.
- Dennis, J.W. et al. (2002) Biochim. Biophys. Acta 1573:414.
- Granovsky, M. et al. (2008) Nat. Med. 7:1.
- Kim, Y.S. et al. (2008) Mol. Cell. Proteomics 7:1.
Long Name
alpha-1,6-Mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Alternate Names
MGAT5, N-Acetylglucosaminyltransferase V
Gene Symbol
MGAT5
UniProt
Additional N-Acetylglucosaminyltransferase V/MGAT5 Products
Product Documents for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
Product Specific Notices for Human N-Acetylglucosaminyltransferase V/MGAT5 Antibody
For research use only
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