Human N-Acetylglucosaminyltransferase V/MGAT5 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB5469N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 706824
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human N-Acetylglucosaminyltransferase V/MGAT5
Leu189-Leu741
Accession # Q09328
Leu189-Leu741
Accession # Q09328
Specificity
Detects human N-Acetylglucosaminyltransferase V/MGAT5 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: N-Acetylglucosaminyltransferase V/MGAT5
N-Acetylglucosaminyltransferase V (GnT-V), also known as mannosylglycoprotein N-acetyl-glucosaminyltransferase 5 (MGAT5), adds an N-acetylglucosamine to the alpha1-6-linked core mannose of an N-linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta1-6GlcNAc-branched arm in N-glycans. The degree of N-glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT-V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3-5).
Long Name
alpha-1,6-Mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Alternate Names
MGAT5, N-Acetylglucosaminyltransferase V
Gene Symbol
MGAT5
UniProt
Additional N-Acetylglucosaminyltransferase V/MGAT5 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Loading...
Loading...
Loading...