Detects human Stathmin-2/STMN2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Stathmin-1, -3, or -4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Mouse Stathmin-2/STMN2 Antibody

Detection of Human Stathmin‑2/STMN2 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Stathmin-1 and recombinant human Stathmin-2 (20 ng/lane) were included. A specific band was detected for Stathmin-2/STMN2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human, Mouse, and Rat Stathmin-2/STMN2 by Western Blot.

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, SH-SY5Y human neuroblastoma cell line, Mouse Brain, and Rat Brain. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Stathmin-2/STMN2 at approximately 21-23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Stathmin‑2/STMN2 in Human Liver.

Stathmin-2/STMN2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse Stathmin-2/STMN2 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

0.05 - 0.2 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line, Mouse Brain, and Rat Brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stathmin-2/STMN2

Stathmin-2 (STMN2), also known as superior cervical ganglion 10 protein (SCG10), is a 19 kDa microtubule-destabilizing protein that is preferentially expressed in neurons. It promotes neuronal proliferation and migration as well as neurite outgrowth. Stathmin-2 contains a membrane attachment region
(aa 1‑26), a regulatory domain (aa 39-96), and an overlapping coiled-coil domain (aa 75-179). It is subject to phosphorylation of multiple serines within the regulatory domain. Within aa 26‑179, human Stathmin-2 shares 100% and 99% aa sequence identity with mouse and rat Stathmin-2, respectively.

Alternate Names

SCG10, SCGN10, Stathmin2, STMN2

Entrez Gene IDs

11075 (Human); 20257 (Mouse); 84510 (Rat)

Gene Symbol

STMN2

UniProt

Q93045

Additional Stathmin-2/STMN2 Products

Product Documents for Human/Mouse Stathmin-2/STMN2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Stathmin-2/STMN2 Antibody

For research use only

Citations
Reviews

Human/Mouse Stathmin-2/STMN2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6930

Key Product Details

Species Reactivity

Validated:

Cited:

Applications