Human/Mouse SOX2 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC2018A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 245610
Product Specifications
Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Gly135-Met317
Accession # P48431
Specificity
Detects human and mouse SOX2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse SOX2 APC-conjugated Antibody
Detection of SOX2 in NTera-2 Human Cell Line by Flow Cytometry.
NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SOX2 APC-conjugated Monoclonal Antibody (Catalog # IC2018A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse SOX2 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: NTera‑2 human testicular embryonic carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Reviewed Applications
Read 1 review rated 4 using IC2018A in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SOX2
References
- Graham, V. et al. (2003) Neuron 39:749.
- Avilion, A.A. et al. (2003) Genes Dev. 17:126.
- Kishi, M. et al. (2000) Development 127:791.
- Yuan, H. et al. (1995) Genes Dev. 9:2635.
- Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
- Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name
Transcription Factor SOX2
Alternate Names
ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2
Entrez Gene IDs
6657 (Human)
Gene Symbol
SOX2
UniProt
Additional SOX2 Products
Product Documents for Human/Mouse SOX2 APC-conjugated Antibody
Product Specific Notices for Human/Mouse SOX2 APC-conjugated Antibody
For research use only
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