Human/Mouse SOX2 Alexa Fluor® 488-conjugated Antibody

Name: Human/Mouse SOX2 Alexa Fluor® 488-conjugated Antibody
Brand: R&D Systems
SKU: IC2018G
Price: 376 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # IC2018G

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Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	E. coli-derived recombinant human SOX2 Gly135-Met317 Accession # P48431
Applications:	Flow Cytometry
Label:	Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)
Source:	Monoclonal Mouse IgG_2A Clone # 245610
Specificity:	Detects human and mouse SOX2 in Western blots.

View Full Technical Details for Human/Mouse SOX2 Alexa Fluor® 488-conjugated Antibody

Applications

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	5 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples

	Intracellular Staining by Flow Cytometry Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SOX2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC2018G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).

Item	Value
References	Graham, V. et al. (2003) Neuron 39:749. Avilion, A.A. et al. (2003) Genes Dev. 17:126. Kishi, M. et al. (2000) Development 127:791. Yuan, H. et al. (1995) Genes Dev. 9:2635. Uwanogho, D. et al. (1995) Mech. Dev. 49:23. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name	Transcription Factor SOX2
Entrez Gene IDs	6657 (Human)
Alternate Names	ANOP3, MCOPS3, MGC2413, SOX2, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX-2, transcription factor SOX2

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human/Mouse SOX2 Alexa Fluor® 488-conjugated Antibody

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Human/Mouse SOX2 Alexa Fluor® 488-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications

Scientific Data Examples

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: SOX2

Product Specific Notices

Citations for Human/Mouse SOX2 Alexa Fluor® 488-conjugated Antibody

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