Human/Mouse Serum Albumin Antibody
Catalog # AF3329 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human/Mouse Serum Albumin Antibody
Immunogen
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse Serum Albumin Antibody
Detection of Human and Mouse Serum Albumin by Western Blot.
Western blot shows lysates of human liver tissue and mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Serum Albumin in HepG2 Human Cell Line.
Serum Albumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Detection of Human and Mouse Albumin by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Serum Albumin Antibody
Immunocytochemistry
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Simple Western
Sample: Human liver tissue, mouse liver tissue, hepatocyte differentiated iBJ6 human induced pluripotent stem cell line, and human serum
Western Blot
Sample: Human liver tissue and mouse liver tissue
Published Applications
Read 4 publications using AF3329 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Albumin
Product Documents for Human/Mouse Serum Albumin Antibody
Product Specific Notices for Human/Mouse Serum Albumin Antibody
For research use only
Citations for Human/Mouse Serum Albumin Antibody (4)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Serum Albumin Antibody include:
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Species: Mouse
Sample Types: Whole Tissue
Applications: IHCF Alonso et al. (2022-09-20), Fibrillin-1 Regulates Arteriole Integrity in the Retina Biomolecules, 2022-012(10).
PMID: 36291539 -
Species: Mouse,Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHCSJ Park et al. (2022-08-22), Blocking CHOP-dependent TXNIP shuttling to mitochondria attenuates albuminuria and mitigates kidney injury in nephrotic syndrome Proceedings of the National Academy of Sciences of the United States of America, 2022-0119(35):e2116505119.
PMID: 35994650 -
Species: Mouse,Transgenic Mouse,Human
Sample Types: Whole Cells,Whole Tissue
Applications: IHC,ICCI Hwang et al. (2021-01-06), Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis Cell & bioscience, 2021-011(1):3.
PMID: 33407858 -
Species: Mouse
Sample Types: Plasma
Applications: Functional Assay(2016-09-21), Opposing impacts on healthspan and longevity by limiting dietary selenium in telomere dysfunctional mice Aging Cell, 2016-00(0).
PMID: 27653523
There are no citations that match your criteria.
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