Human/Mouse Serum Albumin Antibody

Name: Human/Mouse Serum Albumin Antibody
Brand: R&D Systems
SKU: AF3329

Catalog # AF3329 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human/Mouse Serum Albumin Antibody

Immunogen

Mouse Serum Albumin

Specificity

Detects human and mouse Serum Albumin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Serum Albumin Antibody

Detection of Human and Mouse Serum Albumin by Western Blot.

Western blot shows lysates of human liver tissue and mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Serum Albumin in HepG2 Human Cell Line.

Serum Albumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human and Mouse Albumin by Simple Western^TM.

Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Applications for Human/Mouse Serum Albumin Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Simple Western

5-10 µg/mL
Sample: Human liver tissue, mouse liver tissue, hepatocyte differentiated iBJ6 human induced pluripotent stem cell line, and human serum

Western Blot

0.1 µg/mL
Sample: Human liver tissue and mouse liver tissue

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using AF3329 in the following applications:

Functional Assay (1 Publication)
ICC (1 Publication)
IHC (3 Publications)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Albumin

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Alternate Names

ALB

Entrez Gene IDs

213 (Human); 11657 (Mouse); 24186 (Rat)

Gene Symbol

ALB

Product Documents for Human/Mouse Serum Albumin Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse Serum Albumin Antibody

For research use only

Citations for Human/Mouse Serum Albumin Antibody (4)

Showing 1 - 4 of 4 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Serum Albumin Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

F Alonso et al. (2022-09-20), Fibrillin-1 Regulates Arteriole Integrity in the Retina Biomolecules, 2022-012(10).
PMID: 36291539
Species: Mouse,Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC

SJ Park et al. (2022-08-22), Blocking CHOP-dependent TXNIP shuttling to mitochondria attenuates albuminuria and mitigates kidney injury in nephrotic syndrome Proceedings of the National Academy of Sciences of the United States of America, 2022-0119(35):e2116505119.
PMID: 35994650
Species: Mouse,Transgenic Mouse,Human
Sample Types: Whole Cells,Whole Tissue
Applications: IHC,ICC

I Hwang et al. (2021-01-06), Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis Cell & bioscience, 2021-011(1):3.
PMID: 33407858
Species: Mouse
Sample Types: Plasma
Applications: Functional Assay

(2016-09-21), Opposing impacts on healthspan and longevity by limiting dietary selenium in telomere dysfunctional mice Aging Cell, 2016-00(0).
PMID: 27653523

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