Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6006C
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
PerCP (Excitation = 482 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 600380
Product Specifications
Immunogen
Human ROR gammat peptide
Met1-Arg10
Accession # P51449
Met1-Arg10
Accession # P51449
Specificity
Detects human and mouse ROR gammat/RORC2 in direct ELISAs. In direct ELISAs, no cross-reactivity with human and mouse RORC.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Antibody
Detection of ROR gammat/RORC2/NR1F3 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of ROR gammat/RORC2/NR1F3 in Mouse Thymocytes by Flow Cytometry.
Mouse thymocytes were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL‑23 (Catalog # 1290-IL) and mouse thymocytes were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL‑23 (Catalog # 1290-IL) and mouse thymocytes were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR gamma t/RORC2/NR1F3
Long Name
Retinoic Acid-Related Orphan Receptor gamma t
Alternate Names
RORC2
Entrez Gene IDs
6097 (Human)
Gene Symbol
RORC
UniProt
Additional ROR gamma t/RORC2/NR1F3 Products
Product Documents for Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Antibody
Product Specific Notices for Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...