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Recombinant Human IL-23 Protein

Catalog # 1290-IL | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1290-IL-010/CF
1290-IL-500/CF

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With Carrier
1290-IL-010

Key Product Details

Source

Sf 21 (baculovirus)

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-23 protein
Human IL-23 p40
(Ile23-Ser328)
Accession # P29460
GSGSSRGGSGSGGSGGGGSKL Human IL-23 p19
(Arg20-Pro189)
Accession # AAG37232
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile23 (IL-23 single chain & p40) & Ala21 (p19)

Predicted Molecular Mass

55.0 kDa (IL-23 single chain); 36.5 kDa (p40) & 18.5 kDa (p19)

SDS-PAGE

58-67 kDa, 40-48 kDa and 20 kDa, reducing conditions

Activity

Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
The ED50 for this effect is 0.05-0.3 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1290-IL
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1290-IL/CF
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

References

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Long Name

Interleukin 23

Alternate Names

IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF

Entrez Gene IDs

51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)

Gene Symbol

IL23A

Additional IL-23 Products

Product Documents for Recombinant Human IL-23 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-23 Protein

For research use only

Citations for Recombinant Human IL-23 Protein (114)

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Customer Reviews for Recombinant Human IL-23 Protein (6)

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  • Recombinant Human IL-23 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/07/2020
  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 03/19/2019
  • Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 12/04/2018
  • Recombinant Human IL-23 Protein
    Name: Mohammed Sadat
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 12/01/2017
  • Recombinant Human IL-23 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 12/15/2016
    We add to our cell culture as a supplement.
  • Recombinant Human IL-23 Protein
    Name: Anonymous
    Verified Customer | Posted 08/14/2016
    Human Th17 cells were differentiated and maintained.
    Recombinant Human IL-23 Protein 1290-IL

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FAQs for Recombinant Human IL-23 Protein

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  • Q: Does Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF ) have any tags?

    A: Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) does not have any tags.  Please refer to the Source section on the product-specific page for sequence information.

  • Q: Has Recombinant Human IL-23 Protein (Catalog # 1290-IL or Catalog # 1290-IL/CF) been tested for activity on mouse or non-human primate species?

    A: Recombinant Human IL-23 Protein (Catalog # 1290-IL or 1290-IL/CF) activity is measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-0.3 ng/mL.  We have not tested activity of the protein on non-human primate cells in our lab.  Please refer the Citations tab to find publications reporting the use of the protein on other species.  Filter the results based on species and sample type for a more relevant search to your target species.

  • Q: Human IL-23 and Human IL-12 share a p40 subunit.  Why is the predicted molecular weight for the p40 subunit 36.5 kDa in the Recombinant Human IL-23 Protein (Catalog # 1290-IL), while the predicted molecular weight for the p40 subunit is 34.7 kDa for the Recombinant Human IL-12 Protein  (Catalog # 219-IL)?

    A: The Recombinant Human IL-23 Protein (Catalog # 1290-IL) and Recombinant Human IL-12 Protein (Catalog # 219-IL) p40 subunits share the same p40 sequence, Ile23-Ser328 (Accession # P29460).  However, the p40 subunit for Recombinant Human IL-23 (Catalog # 1290-IL) also contains a portion of the GS linker that remains after cleaving p40 subunit from the p19 subunit, so its predicted molecular is slightly larger (36.5 kDa) compared to that of the Recombinant Human IL-12 Protein (Catalog # 219-IL; 34.7 kDa).  Please see the product-specific page for detailed sequence information.

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