Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Talin1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5456) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Talin1 at approximately 250-270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human and Mouse Talin1 by Western Blot.

Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Human/Mouse/Rat Talin1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5456) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Talin1 at approximately 250-270 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 2.

Applications for Human/Mouse/Rat Talin1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, NRK rat normal kidney cells, HeLa human cervical epithelial carcinoma cell line, and BaF3 mouse pro-B cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Talin1

Talin1 (talus; Latin for ankle, the shape of a focal adhesion plaque complex) is a 250‑270 kDa member of the I/LWEQ module superfamily. When activated, it serves as a cytoplasmic, homodimeric link between integrins and vinculin, an actin organizer. Human Talin1 is 2541 amino acids (aa) in length and contains one globular N‑terminal FERM domain that binds integrin cytodomains (aa 86‑403), and a rod shaped C‑terminus (aa 482‑2541) that binds vinculin, and contains an I/LWEQ dimerization domain (aa 2293‑2533). Over amino acids 975‑1181, human Talin1 shares 98% aa identity with mouse Talin1.

Alternate Names

KIAA1027, TLN1

Entrez Gene IDs

7094 (Human); 21894 (Mouse); 313494 (Rat)

Gene Symbol

TLN1

UniProt

Q9Y490 (Human)

Additional Talin1 Products

Product Documents for Human/Mouse/Rat Talin1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat Talin1 Antibody

For research use only

Citations
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