Human/Mouse/Rat SOX2 Antibody

Name: Human/Mouse/Rat SOX2 Antibody
Brand: R&D Systems
SKU: MAB2018R

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB2018R

Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human SOX2 Gly135-Met317 Accession # P48431
Applications:	Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Recombinant Monoclonal Mouse IgG_2A Clone # 245610R
Specificity:	Detects human and mouse SOX2 in Western blots.

View Full Technical Details for Human/Mouse/Rat SOX2 Antibody

Applications for Human/Mouse/Rat SOX2 Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Immunohistochemistry	5-25 µg/mL	Immersion fixed paraffin-embedded sections of glioblastoma
Intracellular Staining by Flow Cytometry	0.25 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat SOX2 Antibody

	Western Blot Detection of Human, Mouse, and Rat SOX2 by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Intracellular Staining by Flow Cytometry Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Recombinant Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018R, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
	Immunohistochemistry Detection of SOX2 in glioblastoma. SOX2 was detected in immersion fixed paraffin-embedded sections of glioblastoma using Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018R) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

Item	Value
References	Graham, V. et al. (2003) Neuron 39:749. Avilion, A.A. et al. (2003) Genes Dev. 17:126. Kishi, M. et al. (2000) Development 127:791. Yuan, H. et al. (1995) Genes Dev. 9:2635. Uwanogho, D. et al. (1995) Mech. Dev. 49:23. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name	Transcription Factor SOX2
Entrez Gene IDs	6657 (Human)
Alternate Names	ANOP3, MCOPS3, MGC2413, SOX2, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX-2, transcription factor SOX2

Citations for Human/Mouse/Rat SOX2 Antibody

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Human/Mouse/Rat SOX2 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human/Mouse/Rat SOX2 Antibody

Scientific Data Examples for Human/Mouse/Rat SOX2 Antibody

Western Blot

Intracellular Staining by Flow Cytometry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: SOX2

Citations for Human/Mouse/Rat SOX2 Antibody

Product Documents for Human/Mouse/Rat SOX2 Antibody

Product Datasheets

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