Human/Mouse/Rat SOX1 Antibody

Name: Human/Mouse/Rat SOX1 Antibody
Brand: R&D Systems
SKU: AF3369
Rating: 5 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF3369

Citations (57)

(4)

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human SOX1 Asn242-Gly379 (Leu276Ile) Accession # NP_005977
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human SOX1 in direct ELISAs and Western blots.

View Full Technical Details for Human/Mouse/Rat SOX1 Antibody

Applications for Human/Mouse/Rat SOX1 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	3-15 µg/mL	Immersion fixed paraffin-embedded sections of human brain (cortex)
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat SOX1 Antibody

	Western Blot Detection of Human SOX1 by Western Blot. Western blot shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry SOX1 in ectoderm differentuiated BG01V Human Embryonic Stem Cells. SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
	Immunocytochemistry SOX1 in differentiated NTera‑2 Human Cell Line. SOX1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunocytochemistry SOX1 in Rat Cortical Stem Cells. SOX1 was detected in immersion fixed rat cortical stem cells (NSC001) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunocytochemistry SOX1 in Mouse Cortical Stem Cells. SOX1 was detected in immersion fixed mouse cortical stem cells (Catalog # NSC002) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry SOX1 in Human Brain (Cortex). SOX1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neuron. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Simple Western Detection of Human SOX1 by Simple Western^TM. Simple Western lane view shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells, loaded at 0.2 mg/mL. A specific band was detected for SOX1 at approximately 50 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX1

SOX1 is a 39 kDa transcription factor that belongs to the SOXB1 subgroup. Within the developing CNS, SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.

Item	Value
Long Name	SRY-related HMG-box 1
Entrez Gene IDs	6656 (Human); 20664 (Mouse)
Alternate Names	SOX1, SRY (sex determining region Y)-box 1, SRY-related HMG-box gene 1, transcription factor SOX-1

Citations for Human/Mouse/Rat SOX1 Antibody (57)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat SOX1 Antibody include:

Species: Primate (Macaca fascicularis)
Sample Types: Whole Tissue
Applications: IHC

X Zhu et al. (2022-01-01), BRN2 as a key gene drives the early primate telencephalon development Science Advances, 8(9):eabl7263.
PMID: 35245119
Species: Human
Sample Types: Whole Cells
Applications: ICC

KG Zyner et al. (2022-01-01), G-quadruplex DNA structures in human stem cells and differentiation Nature Communications, 13(1):142.
PMID: 35013231
Species: Human
Sample Types: Whole Cells
Applications: ICC

HS Meharena et al. (2022-01-01), Down-syndrome-induced senescence disrupts the nuclear architecture of neural progenitors Cell Stem Cell, 29(1):116-130.e7.
PMID: 34995493
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Tisch et al. (2022-01-01), Generation of the human induced pluripotent stem cell line (IBKMOLi002-A) from PBMCs of a patient carrying the heterozygous L271H mutation of the voltage-gated calcium channel subunit Cav1.3-encoding CACNA1D gene Stem Cell Research, 61(0):102784.
PMID: 35453044
Species: Human
Sample Types: Transfected Whole Cells
Applications: ICC

P Mencke et al. (2022-01-01), Generation and characterization of a genetic Parkinson's disease-patient derived iPSC line DJ-1-delP (LCSBi008-A) Stem Cell Research, 62(0):102792.
PMID: 35500378
Species: Human
Sample Types: Organoids
Applications: IHC

M Rhinn et al. (2022-01-01), Aberrant induction of p19Arf-mediated cellular senescence contributes to neurodevelopmental defects PloS Biology, 20(6):e3001664.
PMID: 35700169
Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Zheng et al. (2022-01-01), Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Progenitor Cells Promotes Forelimb Functional Recovery after Cervical Spinal Cord Injury Cells, 11(17).
PMID: 36078173
Species: Human
Sample Types: Organoid
Applications: IHC

MV Brady et al. (2022-01-01), Mispatterning and interneuron deficit in Tourette Syndrome basal ganglia organoids Molecular Psychiatry, 0(0).
PMID: 36447010
Species: Human
Sample Types: Whole Cells
Applications: ICC

H Lin et al. (2022-01-01), Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD) Stem Cell Research, 65(0):102958.
PMID: 36343514
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Li et al. (2022-01-01), Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson's disease carrying a novel heterozygotic PARK8 (LRRK2) mutation Stem Cell Research, 66(0):102995.
PMID: 36528012
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

C Gong et al. (2021-01-01), Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury Cell Reports, 34(12):108889.
PMID: 33761348
Species: Human
Sample Types: Organoid
Applications: IHC

V Krenn et al. (2021-01-01), Organoid modeling of Zika and herpes simplex virus 1 infections reveals virus-specific responses leading to microcephaly Cell Stem Cell, 0(0).
PMID: 33838105
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

KP Kim et al. (2021-01-01), Donor cell memory confers a metastable state of directly converted cells Cell Stem Cell, 0(0).
PMID: 33848472
Species: Human
Sample Types: Whole Cells
Applications: ICC

DM Dykxhoorn et al. (2021-01-01), Derivation of iPSC line UMi029-A bearing a hearing-loss associated variant in the SMPX gene Stem Cell Research, 54(0):102405.
PMID: 34052664
Species: Mouse
Sample Types: Whole Cells
Applications: IHC

T Pachano et al. (2021-01-01), Orphan CpG islands amplify poised enhancer regulatory activity and determine target gene responsiveness Nature Genetics, 53(7):1036-1049.
PMID: 34183853
Species: Human
Sample Types: Whole Cells
Applications: ICC

V Meraviglia et al. (2020-01-01), Generation of human induced pluripotent stem cell line LUMCi027-A and its isogenic gene-corrected line from a patient affected by arrhythmogenic cardiomyopathy and carrying the c.2013delC PKP2 mutation Stem Cell Res, 46(0):101835.
PMID: 32485643
Species: Human
Sample Types: Whole Cells
Applications: ICC

V Meraviglia et al. (2020-01-01), Generation of two human induced pluripotent stem cell lines, LUMCi020-A and LUMCi021-A, from two patients with Catecholaminergic Polymorphic Ventricular Tachycardia carrying heterozygous mutations in the RYR2 gene Stem Cell Res, 45(0):101764.
PMID: 32315959
Species: Human
Sample Types: Whole Cells
Applications: ICC

A Lundin et al. (2020-01-01), Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery Nat Commun, 11(1):4903.
PMID: 32994412
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Malankhano et al. (2020-01-01), A Human Induced Pluripotent Stem Cell-Derived Isogenic Model of Huntington's Disease Based on Neuronal Cells Has Several Relevant Phenotypic Abnormalities J Pers Med, 10(4).
PMID: 33182269
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

JM Dias et al. (2020-01-01), A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells Science Advances, 6(38).
PMID: 32938678
Species: Human
Sample Types: Whole Cells
Applications: ICC

K Hirata et al. (2020-01-01), 4-Phenylbutyrate ameliorates apoptotic neural cell death in Down syndrome by reducing protein aggregates Sci Rep, 10(1):14047.
PMID: 32820178
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Martins et al. (2020-01-01), IPSC-Derived Neuronal Cultures Carrying the Alzheimer's Disease Associated TREM2 R47H Variant Enables the Construction of an A&beta-Induced Gene Regulatory Network Int J Mol Sci, 21(12).
PMID: 32630447
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Park et al. (2019-01-01), Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23) Stem Cell Res, 39(0):101499.
PMID: 31352199
Species: Human
Sample Types: Whole Cells
Applications: ICC

B Di Stefano et al. (2019-01-01), The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis Cell Stem Cell, 0(0).
PMID: 31588046
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

P Wörsdörfer et al. (2019-01-01), Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells Sci Rep, 9(1):15663.
PMID: 31666641
Species: Human
Sample Types: Whole Cells
Applications: ICC

N Bredenkamp et al. (2019-01-01), Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency Stem Cell Reports, 13(6):1083-1098.
PMID: 31708477
Species: Human
Sample Types: Whole Tissue
Applications: IHC

M Huang et al. (2019-01-01), Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis Cell Stem Cell, 0(0).
PMID: 31204176
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Chanda et al. (2019-01-01), Direct Reprogramming of Human Neurons Identifies MARCKSL1 as a Pathogenic Mediator of Valproic Acid-Induced Teratogenicity Cell Stem Cell, 25(1):103-119.e6.
PMID: 31155484
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Zhang et al. (2018-01-01), Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells Stem Cell Res Ther, 9(1):67.
PMID: 29544541
Species: Human
Sample Types: Whole Cells
Applications: ICC

L Marrone et al. (2018-01-01), Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease PLoS ONE, 13(3):e0192497.
PMID: 29513666
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

WA Pastor et al. (2018-01-01), TFAP2C regulates transcription in human naive pluripotency by opening enhancers Nat. Cell Biol., 20(5):553-564.
PMID: 29695788
Species: Human
Sample Types: Whole Cells
Applications: ICC

JY Kim et al. (2018-01-01), Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells Exp. Mol. Med., 50(4):24.
PMID: 29650976
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

CV Latchouman et al. (2018-01-01), Chronic Electrical Stimulation Promotes the Excitability and Plasticity of ESC-derived Neurons following Glutamate-induced Inhibition In vitro Sci Rep, 8(1):10957.
PMID: 30026496
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

NS Corsini et al. (2018-01-01), Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation Cell Stem Cell, 22(4):543-558.e12.
PMID: 29625069
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

AM Lager et al. (2018-01-01), Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells Nat Commun, 9(1):3708.
PMID: 30213958
Species: Human
Sample Types: Whole Cells
Applications: ICC

AD Senft et al. (2018-01-01), Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming Cell Rep, 24(8):1977-1985.e7.
PMID: 30134160
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Li et al. (2018-01-01), A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family PLoS ONE, 13(12):e0208110.
PMID: 30540809
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Cells
Applications: ICC

Y Kang et al. (2018-01-01), Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys Cell Rep, 25(9):2563-2576.e9.
PMID: 30485820
Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Li et al. (2017-01-01), Transcriptome analysis reveals determinant stages controlling human embryonic stem cell commitment to neuronal cells J. Biol. Chem., 292(48):19590-19604.
PMID: 28972157
Species: Mouse
Sample Types: Whole Cells
Applications: IHC

Rui Gao (2017-01-01), Direct induction of neural progenitor cells transiently passes through a partially reprogrammed state Biomaterials, 119(0):53-67.
PMID: 28006658
Species: Human
Sample Types: Whole Cells
Applications: ICC

M Bershteyn et al. (2017-01-01), Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia Cell Stem Cell, 0(0).
PMID: 28111201
Species: Human
Sample Types: Whole Cells
Applications: ICC

X Yuan et al. (2017-01-01), A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model PLoS ONE, 12(4):e0174074.
PMID: 28441409
Species: Human
Sample Types: Whole Cells
Applications: ICC

P Walsh et al. (2017-01-01), Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells Cell Transplant, 26(12):1890-1902.
PMID: 29390875
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Yamashita et al. (2017-01-01), Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells PLoS ONE, 12(2):e0171947.
PMID: 28192470
Species: Human
Sample Types: Whole Tissue
Applications: IHC

L Subramania et al. (2017-01-01), Dynamic behaviour of human neuroepithelial cells in the developing forebrain Nat Commun, 8(0):14167.
PMID: 28139695
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

(2016-01-01), Cytosine-5 RNA Methylation Regulates Neural Stem Cell Differentiation andï¿½Motility Stem Cell Reports, 0(0).
PMID: 28041877
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

(2016-01-01), Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A Stem Cells Transl Med, 0(0).
PMID: 27484861
Species: Mouse
Sample Types: Whole Cells
Applications: IHC

Paull D et al. (2015-01-01), Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Nat Methods, 12(9):885-92.
PMID: 26237226
Species: Human
Sample Types: Whole Cells
Applications: IHC

Nagaoka M et al. (2015-01-01), Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells. PLoS ONE, 10(8):e0136350.
PMID: 26308339
Species: Human
Sample Types: Whole Cells
Applications: ICC

Ferrarese R et al. (2014-01-01), Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest, 124(7):2861-76.
PMID: 24865424
Species: Human
Sample Types: Whole Cells
Applications: ICC

Villa-Diaz L et al. (2014-01-01), Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates. Stem Cells Transl Med, 3(12):1410-7.
PMID: 25313201
Species: Human
Sample Types: Whole Cells
Applications: IHC

Simone C et al. (2014-01-01), iPSC-Derived neural stem cells act via kinase inhibition to exert neuroprotective effects in spinal muscular atrophy with respiratory distress type 1. Stem Cell Reports, 3(2):297-311.
PMID: 25254343
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Najm et al. (2013-01-01), Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol, 31(5):426-33.
PMID: 23584611
Reinhardt P et al. (2013-01-01), Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling. PLoS ONE, 8(3):e59252.
PMID: 23533608
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Vue TY et al. (2009-01-01), Sonic hedgehog signaling controls thalamic progenitor identity and nuclei specification in mice. J. Neurosci., 29(14):4484-97.
PMID: 19357274
Species: Human
Sample Types: Whole Cells
Applications: ICC

Nevin Z et al. (), Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes. Am J Hum Gene100(4):617-634.
PMID: 28366443
Species: Human
Sample Types: Whole Cells
Applications: ICC

Jiang J et al. (), Translating dosage compensation to trisomy 21. Natur500(7462):296-300.
PMID: 23863942

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Customer Reviews for Human/Mouse/Rat SOX1 Antibody (4)

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Human/Mouse/Rat SOX1 Antibody

Name: Ying Chen

Application: ICC/IF

Sample Tested: neural stem cells derived from h9

Species: Human

Verified Customer | Posted 08/19/2019

Neural stem cells derived from H9 cells were fixed in 4% PFA for 15 minutes. The cells were incubated in the sox1 antibody at the concentration of 5ug/ml overnight at 4 degree, the followed with secondary antibody incubation at room temperature for 1hour.
Human/Mouse/Rat SOX1 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: hESC-derived NSC

Species: Human

Verified Customer | Posted 03/21/2018

SOX1 was detected human embryonic stem cells-derived neural stem cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 1 µg/mL for overnight at 4°C. Cells were stained using the Donkey anti-Goat IgG (H+L) Cross-Adsorbed, Alexa Fluor® 568 and counterstained with DAPI (blue).
Human SOX1 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: human NSC

Species: Human

Verified Customer | Posted 10/26/2015

Specificity: SpecificSensitivity: SensitiveBuffer: 1% BSA + 0.3% Triton X-100 in PBSDilution: 1/100
Human SOX1 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: human fibroblast and human neural stem cells

Species: Human

Verified Customer | Posted 10/26/2015

Specificity: SpecificSensitivity: SensitiveBuffer: 1% BSA + 0.3% Triton X-100 in PBSDilution: 1/100

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Product Documents for Human/Mouse/Rat SOX1 Antibody

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Human/Mouse/Rat SOX1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human/Mouse/Rat SOX1 Antibody

Scientific Data Examples for Human/Mouse/Rat SOX1 Antibody

Western Blot

Immunocytochemistry

Immunocytochemistry

Immunocytochemistry

Immunocytochemistry

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: SOX1

Citations for Human/Mouse/Rat SOX1 Antibody (57)

Customer Reviews for Human/Mouse/Rat SOX1 Antibody (4)

Customer Images

Human/Mouse/Rat SOX1 Antibody

Human/Mouse/Rat SOX1 Antibody

Human SOX1 Antibody

Human SOX1 Antibody

Product Documents for Human/Mouse/Rat SOX1 Antibody

Product Datasheets

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