Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Name: Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody
Brand: R&D Systems
SKU: MAB1205
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1205

Citations (2)

(2)

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Recombinant Monoclonal Antibody.

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	Phosphopeptide containing human, rat, and mouse JNK1 T183/Y185 site
Applications:	Immunocytochemistry/Immunofluorescence, Simple Western, Western Blot
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 1006A
Specificity:	Detects human, mouse and rat p46 and p54 JNK when dually phosphorylated at sites homologous to T183/Y185 of JNK1 and JNK2, and T221/Y223 of JNK3 in Western blots.

View Full Technical Details for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Applications for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10-25 µg/mL	See below
Immunocytochemistry	10-35 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

	Western Blot Detection of Human and Mouse Phospho-JNK (T183/Y185) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and 293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm²ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry Phospho-JNK (T183/Y185) in HEK293 Human Cell Line. JNK phosphorylated at T183/Y185 was detected in immersion fixed HEK293 human embryonic kidney cell line untreated (lower panel) or treated with UV radiation (upper panel) using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI. Filamentous actin was stained with fluorescein-conjugated phalloidin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Simple Western Detection of Human Phospho-JNK (T183/Y185) by Simple Western^TM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 J/m²ultraviolet light (UV) followed by a 30 minute recovery, loaded at 0.2 mg/mL. A specific band was detected for Phospho-JNK (T183/Y185) at approximately 46 and 56 kDa (as indicated) using 20 μg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: JNK

The c-Jun N-terminal Kinases (JNKs) are part of the MAPK (mitogen-activated protein kinase) system that transmits signals from the extracellular milieu to both the cytoplasm and nucleus of the cell. Following perturbation at the cell membrane, MEKKs/MAP3Ks are initially activated, followed by their activation of MKKs/MAP2Ks, and MKKs activation of MAPKs/MAP(1)Ks. There are three classes of MAPKs: ERKs, p38 Kinases and JNKs. JNKs are 45-55 kDa protein products of three genes which, through alternative splicing, generate up to 10 possible isoforms. The phosphorylation targets for MAPKs vary, but include p53, c-MYC, ATF2 and c-Jun, the latter molecule representing the namesake for the enzyme group. The three human JNKs share approximately 80% aa sequence identity. JNKs from human, mouse and rat all contain a conserved Met-Met-Thr(183)-Pro-Tyr(185)-Val-Val motif that undergoes dual phosphorylation by MMK4 and MMK7 to activate the different JNKs. Activated by environmental stresses and inflammatory cytokines, JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

Long Name

C-Jun N-terminal Kinase

Alternate Names

EC 2.7.11, EC 2.7.11.24, JNK, JNK-46, JNK1 alpha protein kinase, JNK1 beta protein kinase, JNK1JNK1A2, JUN N-terminal kinase, MAP kinase 8, MAPK 8, PRKM8JNK, SAPK1JNK21B1/2, Stress-activated protein kinase 1, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, protein kinase JNK1

Citations for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Ö Boyac?o?lu et al. (2021), ACPA decreases non-small cell lung cancer line growth through Akt/PI3K and JNK pathways in vitro Cell Death Disease, 2021;12(1):56.
PMID: 33431819
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

M Tabarsa et al. (2020), Isolation, structural elucidation and immuno-stimulatory properties of polysaccharides from Cuminum cyminum Carbohydr Polym, 230(0):115636.
PMID: 31887877

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Customer Reviews for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody (2)

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Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Name: Anonymous

Application: WB

Sample Tested: HeLa human cervical epithelial carcinoma cell line

Species: Human

Verified Customer | Posted 11/03/2021
Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Name: Ryan Patel

Application: ChIP

Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line

Species: Cynomolgus Monkey

Verified Customer | Posted 08/01/2017

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Product Documents for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

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Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

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Applications for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Scientific Data Examples for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Western Blot

Immunocytochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: JNK

Citations for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody (2)

Customer Reviews for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody (2)

Customer Images

Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Product Documents for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Product Datasheets

COA

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