Human/Mouse/Rat Peroxiredoxin 5 Alexa Fluor™ Plus 680-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5724AFP680

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AF5724AFP680-100UG
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Goat IgG

View all Peroxiredoxin 5 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Peroxiredoxin 5

Specificity

Detects human, mouse and rat Peroxiredoxin 5 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Peroxiredoxin 5

Peroxiredoxin 5 (Prx-5; also known as AOEB166 and thioredoxin reductase) is a 22 kDa, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The mouse precursor molecule is 210 amino acids (aa) in length and contains an N-terminal 48 aa mitochondrial targeting (signal) sequence and a 162 aa mature enzyme that shows an NES between aa 54‑63, and a peroxisome targeting motif at aa 208‑210. There are two catalytic cysteines, one at Cys96, and another at Cys200 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H2O2 and alkyl hydroperoxides. Two potential splice forms are reported. One is 17 kDa in size and shows an alternative start site at Met49, while a second shows a 19 aa substitution for aa 83‑102. Mouse Prx-5 is 78% and 90% aa identical to human and rat Prx-5, respectively.

Alternate Names

ACR1, AOEB166, AOPP, PMP20, PRDX5, PRXV

Entrez Gene IDs

25824 (Human); 54683 (Mouse); 113898 (Rat)

Gene Symbol

PRDX5

UniProt

P99029

Additional Peroxiredoxin 5 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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